Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 254232..254818 Replicon chromosome
Accession NZ_CP055109
Organism Shigella flexneri strain SWHIN_104

Toxin (Protein)


Gene name hicA Uniprot ID A0A0H0RQZ4
Locus tag HUZ82_RS01155 Protein ID WP_042014162.1
Coordinates 254232..254462 (+) Length 77 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag HUZ82_RS01160 Protein ID WP_000593555.1
Coordinates 254459..254818 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ82_RS01145 (250269) 250269..253004 + 2736 WP_040091132.1 ribosome-associated ATPase/putative transporter RbbA -
HUZ82_RS01150 (253004) 253004..254128 + 1125 WP_001216257.1 ABC transporter permease -
HUZ82_RS01155 (254232) 254232..254462 + 231 WP_042014162.1 type II toxin-antitoxin system HicA family toxin Toxin
HUZ82_RS01160 (254459) 254459..254818 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
HUZ82_RS01165 (254938) 254938..255339 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
HUZ82_RS01170 (255345) 255345..256151 - 807 WP_000173666.1 nickel import ATP-binding protein NikE -
HUZ82_RS01175 (256148) 256148..256912 - 765 WP_001136236.1 nickel import ATP-binding protein NikD -
HUZ82_RS01180 (256912) 256912..257745 - 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
HUZ82_RS01185 (257742) 257742..258686 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(15-65)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 77 a.a.        Molecular weight: 8419.79 Da        Isoelectric Point: 8.5518

>T161545 WP_042014162.1 NZ_CP055109:254232-254462 [Shigella flexneri]
MDQLFKSPLPQGIKWTDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP

Download         Length: 231 bp

>T161545 NZ_CP071216:1603057-1603365 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGCAATTTACCGTATATGACAATACGTATCCGGCATCAGCCTATCCCTACTTACTGGACGTTCAAAGCGATCTTATTGA
TGTCCTCTCGACCCGGTTAATGATCCCTCTTTATGCTTTGGATAATGTCAGGGTTAAAATTTCTGCACGTTTATGCCCGG
AGATTGAGGTCAACGGCGAAAAGTTTCTTGTTATGACGCATGAGATGGCTGCCGTACGTATTTCGCAGATTGGGAAGGCC
GTTGGTAATGTCAATGAGCACAGGAATCAAATTAAAGCCGCCATCGATTTTCTGATTGATGGTTTTTGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT161545 WP_000593555.1 NZ_CP055109:254459-254818 [Shigella flexneri]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT161545 NZ_CP071216:1602818-1603054 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGGCAAATAACAATGTGACGAAAAAGACAGTCAACGTAACCATCGATCGCGATCTTTTCCAGCGCGCAAAATCCCTGGG
CGTCAATGTGTCATCTGTATTGACGGACGCATTGAGCGTCCGTGTCAGGGACATTGAGATTCAACAGTGGCGCGAGCAAA
ATCGTGCGGCGTTAGAAGAACTTAATCGTATCACTGAAGAAAATGGTTTGTTGTCTGATGAATATCGGACATTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H0RQZ4

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References