Detailed information of TA system TA161544

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 23042..23685
• Replicon: plasmid unnamed2
• Accession: NZ_CP055108
• Organism: Klebsiella pneumoniae strain SWHIN_106

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: B1LRW4
• Locus tag: HUZ83_RS27000
• Protein ID: WP_001044768.1
• Coordinates: 23042..23458 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D2WFK3
• Locus tag: HUZ83_RS27005
• Protein ID: WP_001261287.1
• Coordinates: 23455..23685 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ83_RS26985 (HUZ83_26900)	19541..20131	-	591	WP_000194575.1	hypothetical protein	-
HUZ83_RS26990 (HUZ83_26905)	20131..20388	-	258	WP_000343085.1	hypothetical protein	-
HUZ83_RS26995 (HUZ83_26910)	20742..22880	+	2139	WP_000350638.1	AAA family ATPase	-
HUZ83_RS27000 (HUZ83_26915)	23042..23458	-	417	WP_001044768.1	type II toxin-antitoxin system VapC family toxin	Toxin
HUZ83_RS27005 (HUZ83_26920)	23455..23685	-	231	WP_001261287.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
HUZ83_RS27010 (HUZ83_26925)	23981..24253	+	273	WP_061602529.1	hypothetical protein	-
HUZ83_RS27015 (HUZ83_26930)	24300..25004	+	705	WP_001067858.1	IS6-like element IS26 family transposase	-
HUZ83_RS27020 (HUZ83_26935)	25051..25485	-	435	WP_000429836.1	Hg(II)-responsive transcriptional regulator	-
HUZ83_RS27025 (HUZ83_26940)	25557..25907	+	351	WP_001294663.1	mercuric transport protein MerT	-
HUZ83_RS27030 (HUZ83_26945)	25921..26196	+	276	WP_000732292.1	mercury resistance system periplasmic binding protein MerP	-
HUZ83_RS27035 (HUZ83_26950)	26232..26654	+	423	WP_001340589.1	organomercurial transporter MerC	-
HUZ83_RS27040 (HUZ83_26955)	26706..28400	+	1695	WP_000105636.1	mercury(II) reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	aph(3')-Ia / mef(B) / sul3 / ant(3'')-Ia / cmlA1 / floR / dfrA14 / blaOXA-10 / ARR-3 / qnrS1 / tet(A)	-	1..105546	105546
	-	flank	IS/Tn	-	-	24300..25004	704

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15197.66 Da
• Isoelectric Point: 7.7805

>T161544 WP_001044768.1 NZ_CP055108:c23458-23042 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Nucleotide

• Length: 417 bp

>T161544 NZ_CP071216:c1109291-1109088 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGACTAAAACCGACTATCTGATGCGTTTACGTAAATGCACCACGATTGATACTCTCGAACGTGTTATTGAGAAAAATAA
GTACGAGCTTTCCGATGATGAACTGGAGCTGTTTTACTCAGCGGCCGATCACCGTCTGGCAGAGCTCACCATGAACAAAC
TGTATGACAAAATCCCCACCTCCGTTTGGAAATATGTCAGATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8815.90 Da
• Isoelectric Point: 4.3775

>AT161544 WP_001261287.1 NZ_CP055108:c23685-23455 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT161544 NZ_CP071216:c1109709-1109341 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGGATGAGTATTCGCCTAAGCGGCACGACATTGCTCAGCTCAAATTCTTGTGCGAAAATTTGTATGATGAAGGTATCGC
TACCCTGGGAGACAGTCATCATGGGTGGGTCAACGATCCGACGTCCTCCGTCAACCTGCAGCTTAATGAGTTGATCGAAC
ATATCGCTTCGTTTGTGATGAGTTATAAAATTAAATACATGGACGAATCTGACCTGTCGGAGTTAGTCGAAGAATATCTC
GACGATACCTATACGCTGTTCAGCAGCTATGGTATCAATGATTCCGACCTGCGCCGCTGGCAAAAAACCAAGGCGAGATT
ATTCAGAATGTTCTCAGGAGAGGACATCTGTACGACAATGAAAACTTAG

Structures

Toxin

• AlphaFold DB: A0A606Q844

Antitoxin

• AlphaFold DB: A0A7U9QFC4