Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 23042..23685 Replicon plasmid unnamed2
Accession NZ_CP055108
Organism Klebsiella pneumoniae strain SWHIN_106

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag HUZ83_RS27000 Protein ID WP_001044768.1
Coordinates 23042..23458 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag HUZ83_RS27005 Protein ID WP_001261287.1
Coordinates 23455..23685 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ83_RS26985 (HUZ83_26900) 19541..20131 - 591 WP_000194575.1 hypothetical protein -
HUZ83_RS26990 (HUZ83_26905) 20131..20388 - 258 WP_000343085.1 hypothetical protein -
HUZ83_RS26995 (HUZ83_26910) 20742..22880 + 2139 WP_000350638.1 AAA family ATPase -
HUZ83_RS27000 (HUZ83_26915) 23042..23458 - 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
HUZ83_RS27005 (HUZ83_26920) 23455..23685 - 231 WP_001261287.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
HUZ83_RS27010 (HUZ83_26925) 23981..24253 + 273 WP_061602529.1 hypothetical protein -
HUZ83_RS27015 (HUZ83_26930) 24300..25004 + 705 WP_001067858.1 IS6-like element IS26 family transposase -
HUZ83_RS27020 (HUZ83_26935) 25051..25485 - 435 WP_000429836.1 Hg(II)-responsive transcriptional regulator -
HUZ83_RS27025 (HUZ83_26940) 25557..25907 + 351 WP_001294663.1 mercuric transport protein MerT -
HUZ83_RS27030 (HUZ83_26945) 25921..26196 + 276 WP_000732292.1 mercury resistance system periplasmic binding protein MerP -
HUZ83_RS27035 (HUZ83_26950) 26232..26654 + 423 WP_001340589.1 organomercurial transporter MerC -
HUZ83_RS27040 (HUZ83_26955) 26706..28400 + 1695 WP_000105636.1 mercury(II) reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid aph(3')-Ia / mef(B) / sul3 / ant(3'')-Ia / cmlA1 / floR / dfrA14 / blaOXA-10 / ARR-3 / qnrS1 / tet(A) - 1..105546 105546
- flank IS/Tn - - 24300..25004 704


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T161544 WP_001044768.1 NZ_CP055108:c23458-23042 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T161544 NZ_CP071216:c1109291-1109088 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGACTAAAACCGACTATCTGATGCGTTTACGTAAATGCACCACGATTGATACTCTCGAACGTGTTATTGAGAAAAATAA
GTACGAGCTTTCCGATGATGAACTGGAGCTGTTTTACTCAGCGGCCGATCACCGTCTGGCAGAGCTCACCATGAACAAAC
TGTATGACAAAATCCCCACCTCCGTTTGGAAATATGTCAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT161544 WP_001261287.1 NZ_CP055108:c23685-23455 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT161544 NZ_CP071216:c1109709-1109341 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGGATGAGTATTCGCCTAAGCGGCACGACATTGCTCAGCTCAAATTCTTGTGCGAAAATTTGTATGATGAAGGTATCGC
TACCCTGGGAGACAGTCATCATGGGTGGGTCAACGATCCGACGTCCTCCGTCAACCTGCAGCTTAATGAGTTGATCGAAC
ATATCGCTTCGTTTGTGATGAGTTATAAAATTAAATACATGGACGAATCTGACCTGTCGGAGTTAGTCGAAGAATATCTC
GACGATACCTATACGCTGTTCAGCAGCTATGGTATCAATGATTCCGACCTGCGCCGCTGGCAAAAAACCAAGGCGAGATT
ATTCAGAATGTTCTCAGGAGAGGACATCTGTACGACAATGAAAACTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References