Detailed information of TA system TA161541

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): stbDE/ParE-YafN
• Location: 210937..211459
• Replicon: plasmid unnamed1
• Accession: NZ_CP055107
• Organism: Klebsiella pneumoniae strain SWHIN_106

Toxin (Protein)

• Gene name: stbE
• Uniprot ID: -
• Locus tag: HUZ83_RS26460
• Protein ID: WP_239612456.1
• Coordinates: 211175..211459 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: stbD
• Uniprot ID: A0A2J4R0U8
• Locus tag: HUZ83_RS26455
• Protein ID: WP_004181777.1
• Coordinates: 210937..211185 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ83_RS26435 (HUZ83_26360)	206146..206967	-	822	WP_004181772.1	hypothetical protein	-
HUZ83_RS26440 (HUZ83_26365)	207029..207382	-	354	WP_239612454.1	hypothetical protein	-
HUZ83_RS26445 (HUZ83_26370)	207527..208513	-	987	WP_239612455.1	hypothetical protein	-
HUZ83_RS26450 (HUZ83_26375)	208847..210646	-	1800	WP_065892048.1	ATP-dependent helicase	-
HUZ83_RS26455 (HUZ83_26380)	210937..211185	+	249	WP_004181777.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
HUZ83_RS26460 (HUZ83_26385)	211175..211459	+	285	WP_239612456.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HUZ83_RS26465 (HUZ83_26390)	211613..212839	+	1227	WP_239612457.1	transposase	-
HUZ83_RS26470 (HUZ83_26395)	213086..214195	-	1110	WP_239612458.1	DegT/DnrJ/EryC1/StrS family aminotransferase	-
HUZ83_RS26475 (HUZ83_26400)	214188..214646	-	459	WP_239612459.1	NUDIX domain-containing protein	-
HUZ83_RS26480 (HUZ83_26405)	214647..215498	-	852	WP_239612460.1	DMT family transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	pla	1..301800	301800
	-	flank	IS/Tn	-	-	211613..212839	1226

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-82)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10987.78 Da
• Isoelectric Point: 10.6516

>T161541 WP_239612456.1 NZ_CP055107:211175-211459 [Klebsiella pneumoniae]
MTYKLAFNESALKEWKKLGHTIREQFKKKLAERLLNPRMPASQLHGRKDQYKIKLRGAGYRLVYSVNDDVVTVTVIGVGK
RENDDVYNLTKHRI

Nucleotide

• Length: 285 bp

>T161541 NZ_CP071214:c4454566-4454312 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGAACAAACGACACCAAAAAACGCTGTCAGATGTGTTTGCCCGGCCTGTCAACGGTTCTATAAAGTGGTCTGATATTGA
GGCGCTTTTTACCGCATTAGGGGCGGAGATTCACGAAAGGGAAGGTTCTAGGATCGCGGTGCTGTTGAAAGGTGAAAAAA
GAGTCTTTCACCGGCCACACCCCAGACCTACCACTGACAAGGGGGCGGTTAACTCCATTCGGATCTGGTTGGATAGCTTA
GGAATAAAACCATGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8925.17 Da
• Isoelectric Point: 3.8209

>AT161541 WP_004181777.1 NZ_CP055107:210937-211185 [Klebsiella pneumoniae]
MAFQVLTTTAASITELKRDPMGTFNAGEGAPVAILNRNEPAFYCVPPALYAQLMELLEDQELGRIADERIGEAVIEVSLD
DL

Nucleotide

• Length: 249 bp

>AT161541 NZ_CP071214:c4454312-4453971 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGAATAACACACTGAAAATTGACGGCCATACGGCCGTCATCAACTTCGACCCTGAAATTGAAATGTTCCGGGGCGAGTT
TGTCGGGCTGAATGGCGGCGCCGACTTCTACGCCTACAGCGTGGACGAGCTGAAGAAAGAAGGCGCGATCTCACTCGCGG
TCTTTCTCGATGAGTGCCATAAAGACGGCATCGAGCCTTACAAGTCGTACAGCGGCAAAGTAACCACCCGCCTGTCGCCG
GAACGCCATCAGGCGTTAGCCATTGCCGCACAGGCCACCGGGCAGTCGATTAATGAACTGCTGAATGAAGGTGTTGATCT
GGTTATCGAAAAGCATTCCTGA

Structures

Antitoxin

• AlphaFold DB: A0A2J4R0U8