Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 118894..119537 Replicon plasmid unnamed1
Accession NZ_CP055107
Organism Klebsiella pneumoniae strain SWHIN_106

Toxin (Protein)


Gene name vagD Uniprot ID Q84A06
Locus tag HUZ83_RS25930 Protein ID WP_000754566.1
Coordinates 118894..119310 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag HUZ83_RS25935 Protein ID WP_001261276.1
Coordinates 119307..119537 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ83_RS25910 (HUZ83_25850) 114202..114867 - 666 WP_023328882.1 LuxR C-terminal-related transcriptional regulator -
HUZ83_RS25915 (HUZ83_25855) 115135..116160 + 1026 WP_001101446.1 IS110 family transposase -
HUZ83_RS25920 (HUZ83_25860) 116455..117423 + 969 Protein_113 IS5 family transposase -
HUZ83_RS25925 (HUZ83_25865) 117741..118766 - 1026 WP_001101446.1 IS110 family transposase -
HUZ83_RS25930 (HUZ83_25870) 118894..119310 - 417 WP_000754566.1 type II toxin-antitoxin system VapC family toxin Toxin
HUZ83_RS25935 (HUZ83_25875) 119307..119537 - 231 WP_001261276.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
HUZ83_RS25940 (HUZ83_25880) 120108..120458 + 351 WP_000493378.1 hypothetical protein -
HUZ83_RS25945 (HUZ83_25885) 120509..121252 + 744 WP_000129823.1 hypothetical protein -
HUZ83_RS25950 (HUZ83_25890) 121249..122022 + 774 WP_239612493.1 site-specific integrase -
HUZ83_RS25955 (HUZ83_25895) 122063..123043 + 981 WP_000019445.1 IS5-like element ISKpn26 family transposase -
HUZ83_RS25960 123222..123314 - 93 Protein_121 cytoplasmic protein -
HUZ83_RS25965 (HUZ83_25900) 123421..123645 - 225 WP_049157091.1 hypothetical protein -
HUZ83_RS25970 (HUZ83_25905) 123656..123868 - 213 WP_049157092.1 hypothetical protein -
HUZ83_RS25975 (HUZ83_25910) 123929..124285 - 357 WP_049157093.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - pla 1..301800 301800
- inside IScluster/Tn - - 112900..123043 10143


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15006.52 Da        Isoelectric Point: 9.2957

>T161539 WP_000754566.1 NZ_CP055107:c119310-118894 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK

Download         Length: 417 bp

>T161539 NZ_CP071214:c3786691-3786302 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGTACATGTTTGATACCAACACCGTCAGCCACTTGTTTCGGCAGCATCCGCAGGTGCTTAACGTCATGGAAAAGCTCCC
GCCTTCTGCCGTATGTATTTCCAGCGTCACGGAGGCGGAACTGCTTTACGGTGTGGCTAAACGGCGGAACAAGGCGTTGC
AAAGCATGGTCGAGGCCTTTCTTGCTGCAGTGACGGTCTATGCATGGGATAGCGAGGCCGCCCGTTGCTACGGTGAAATG
CGGGCAACCATGGAGAGGAAGGGCAAAATCATGGGCGCGATCGATCAACTGATCGCCGCTCACGCGCAGAGCCGTGGCGC
GACGCTGGTGACCAACGATCGCGCATTCGCCATGGTGCCCGGGTTGGCGGTTGAAGACTGGACGCGCTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT161539 WP_001261276.1 NZ_CP055107:c119537-119307 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT161539 NZ_CP071214:c3786958-3786695 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGGAAAGAATCGCAAAGCTGTTTAAAAACGGCAGAAATCAGGCGGTGAGGTTACCGGTGGAATTTGAATTTGATACGGA
TCGTGTCTATATCCGTCGCGATCGGGAGGGCAACGTGATTTTATCCAAATATCCGGCAAAGCCGGACAGCTGGGCACCGC
TGCTGAGCATTATCGCCCAAACGCCGGTGCCGCAAGAATTTCTTGGCGCGGAAGATCGTCAGCAAGGGATGGCAGACCGC
GATCCGTTCTCAGAGGAAGAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A387K1G3

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References