Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5121577..5122202 Replicon chromosome
Accession NZ_CP055106
Organism Klebsiella pneumoniae strain SWHIN_106

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag HUZ83_RS24990 Protein ID WP_002882817.1
Coordinates 5121577..5121960 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag HUZ83_RS24995 Protein ID WP_004150355.1
Coordinates 5121960..5122202 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ83_RS24975 (5118943) 5118943..5119845 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
HUZ83_RS24980 (5119842) 5119842..5120477 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
HUZ83_RS24985 (5120474) 5120474..5121403 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
HUZ83_RS24990 (5121577) 5121577..5121960 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
HUZ83_RS24995 (5121960) 5121960..5122202 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
HUZ83_RS25000 (5122407) 5122407..5123324 + 918 WP_065803683.1 alpha/beta hydrolase -
HUZ83_RS25005 (5123338) 5123338..5124279 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
HUZ83_RS25010 (5124324) 5124324..5124761 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
HUZ83_RS25015 (5124758) 5124758..5125618 - 861 WP_004146232.1 virulence factor BrkB family protein -
HUZ83_RS25020 (5125612) 5125612..5126211 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T161538 WP_002882817.1 NZ_CP055106:c5121960-5121577 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T161538 NZ_CP071214:c3109545-3109147 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGTTCAGTCATATGCTGGACACCAACATTGTTATCTATGTCATCAAGCGCCGGCCGCTGGAAGTGCTGGAGGCATTCAA
TCGCTATGCAGGTAAAATGGTCATTTCGTCGATCACTTATGGCGAATTGGTTCACGGGGTGGAGAAAAGCGCTCGTCCAG
CGGTGAACGCCAGAGTCGTTGAGGATTTTGTCTCCCGATTGGACATTCTCGATTACAGCGCCAAAGCGGCATCGCACTAC
GGCAATATCCGGGCTGCGCTGGAACGACAGGGAACGCCTATTGGCGTAAACGATCTACATATTGCCGGGCATGCGCGCAG
CGAAGGATTGATCCTGGTGACCAATAATCGACGCGAATTTGAGCGCGTTGAGGGTTTGCGGCTGGAAAATTGGTTGTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT161538 WP_004150355.1 NZ_CP055106:c5122202-5121960 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT161538 NZ_CP071214:c3109778-3109545 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGACGTTAGGTTCTGTCTTTACTAATAATCGGACACAGGCTGTACGCCTGCCTGCAGAGGCGCGTTTCCCTGATACCGT
TTCTAAAGTGACCGTTCGCGTAGTGGGTAAAGAGCGCATTCTTGCCCCCCTGGAGAATACCTGGGACAGTTTCTTCTGTG
CGGAAAATGAGGTGAGCGATGATTTTCTCAATGAACGTGCCGGACAGCACCAGCGCGAGCGGGAAGCGTTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References