Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4510457..4511267 Replicon chromosome
Accession NZ_CP055106
Organism Klebsiella pneumoniae strain SWHIN_106

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag HUZ83_RS22070 Protein ID WP_004178461.1
Coordinates 4510457..4510990 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag HUZ83_RS22075 Protein ID WP_002887278.1
Coordinates 4511001..4511267 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ83_RS22065 (4509288) 4509288..4510409 + 1122 WP_004214138.1 cupin domain-containing protein -
HUZ83_RS22070 (4510457) 4510457..4510990 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
HUZ83_RS22075 (4511001) 4511001..4511267 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
HUZ83_RS22080 (4511370) 4511370..4512803 - 1434 WP_023283180.1 Cu(+)/Ag(+) sensor histidine kinase -
HUZ83_RS22085 (4512793) 4512793..4513476 - 684 WP_004214143.1 copper response regulator transcription factor CusR -
HUZ83_RS22090 (4513648) 4513648..4515033 + 1386 WP_023283179.1 efflux transporter outer membrane subunit -
HUZ83_RS22095 (4515051) 4515051..4515395 + 345 WP_021462623.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T161534 WP_004178461.1 NZ_CP055106:c4510990-4510457 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T161534 NZ_CP071214:55329-55604 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGGAATTAAAATGGACGAGTAAGGCGCTATCGGATTTGGCGCGTTTGTTTGAGTTTCTTGCCTTGGCTAACCGCACTGC
GGCAGCGAATTCGGTGAAGGCCTTGGTCGCCGCGCCTAAAGCGTTGCTCGATAATCCTCGCCTGGGTGAAAGACTGGATG
AGTTTTTACCGCAGGAAGTGAGGCGTATTCTGGTCGGCCGTTATGAGATACGTTACCAGGTGCAGCCGGCAACGATTTAT
GTTCTGCGTATTTGGCATACCCGCGAAGAGCGATAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT161534 WP_002887278.1 NZ_CP055106:c4511267-4511001 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT161534 NZ_CP071214:55069-55329 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGAATACGAAAGTCATCACCGCTCATGTGCCGCTGCCTTTGGCTGAAAAGGTGGATGAAATGGCCGCCAAACTTGAACG
TTCACGGGGATGGATAGTGAAGCAAGCCCTCGCCGCCTGGCTTGAGCAAGAGGAAGAACGCCGTCGATTAACGCTGGCGG
CGCTGGCCGATGTCGAACAGCAAAACGTTATCGATCATGAGTCGGTAGAAGCCTGGGCTGCCAGCCTGGACAGTGACGAG
CCGCTGCCGGTACCGCGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References