Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 8822..9414 Replicon plasmid unnamed3
Accession NZ_CP055102
Organism Shigella flexneri strain SWHIN_107

Toxin (Protein)


Gene name hicA Uniprot ID A0A1I9ZJC5
Locus tag HUZ84_RS24875 Protein ID WP_000513532.1
Coordinates 8822..9070 (+) Length 83 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A1I9ZJ49
Locus tag HUZ84_RS24880 Protein ID WP_000681609.1
Coordinates 9067..9414 (+) Length 116 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ84_RS24835 (HUZ84_24620) 3911..4576 - 666 WP_000532149.1 division plane positioning ATPase MipZ -
HUZ84_RS24840 (HUZ84_24625) 5174..5503 + 330 WP_000855121.1 hypothetical protein -
HUZ84_RS24845 (HUZ84_24630) 6082..6405 + 324 WP_000427402.1 hypothetical protein -
HUZ84_RS24850 (HUZ84_24635) 6446..6655 + 210 WP_000866036.1 hypothetical protein -
HUZ84_RS24855 (HUZ84_24640) 6752..6976 + 225 WP_000814381.1 hypothetical protein -
HUZ84_RS24860 (HUZ84_24645) 7028..7288 + 261 WP_000702247.1 hypothetical protein -
HUZ84_RS24865 (HUZ84_24650) 7278..7529 + 252 WP_001132407.1 transcriptional regulator -
HUZ84_RS24870 (HUZ84_24655) 8079..8357 + 279 WP_000545938.1 hypothetical protein -
HUZ84_RS24875 (HUZ84_24660) 8822..9070 + 249 WP_000513532.1 type II toxin-antitoxin system HicA family toxin Toxin
HUZ84_RS24880 (HUZ84_24665) 9067..9414 + 348 WP_000681609.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
HUZ84_RS24885 9502..9945 - 444 WP_023223351.1 hypothetical protein -
HUZ84_RS24890 (HUZ84_24675) 10186..10485 + 300 WP_034169410.1 type II toxin-antitoxin system HicB family antitoxin -
HUZ84_RS24895 (HUZ84_24680) 10745..11071 - 327 WP_000206697.1 hypothetical protein -
HUZ84_RS24900 (HUZ84_24685) 11073..11411 - 339 WP_029797038.1 hypothetical protein -
HUZ84_RS24905 11391..11513 - 123 Protein_20 hypothetical protein -
HUZ84_RS24910 (HUZ84_24690) 11533..12240 - 708 WP_000108794.1 hypothetical protein -
HUZ84_RS24915 (HUZ84_24695) 12242..12994 - 753 WP_000797966.1 conjugal transfer protein TraL -
HUZ84_RS24920 (HUZ84_24700) 13012..13413 - 402 WP_021575837.1 hypothetical protein -
HUZ84_RS24925 (HUZ84_24705) 13782..14123 + 342 WP_000730722.1 plasmid mobilization relaxosome protein MobC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - - 1..58696 58696


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(20-81)

Antitoxin

Uncharacterised protein family HicB

(54-104)


Sequences

Toxin        


Download         Length: 83 a.a.        Molecular weight: 9378.03 Da        Isoelectric Point: 10.4800

>T161521 WP_000513532.1 NZ_CP055102:8822-9070 [Shigella flexneri]
MGKADKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
VL

Download         Length: 249 bp

>T161521 NZ_CP071210:c3786712-3786323 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGTACATGTTTGATACCAACACCGTCAGCCACTTGTTTCGGCAGCATCCGCAGGTGCTTAACGTCATGGAAAAGCTCCC
GCCTTCTGCCGTATGTATTTCCAGCGTCACGGAGGCGGAACTGCTTTACGGTGTGGCTAAACGGCGGAACAAGGCGTTGC
AAAGCATGGTCGAGGCCTTTCTTGCTGCAGTGACGGTCTATGCATGGGATAGCGAGGCCGCCCGTTGCTACGGTGAAATG
CGGGCAACCATGGAGAGGAAGGGCAAAATCATGGGCGCGATCGATCAACTGATCGCCGCTCACGCGCAGAGCCGTGGCGC
GACGCTGGTGACCAACGATCGCGCATTCGCCATGGTGCCCGGGTTGGCGGTTGAAGACTGGACGCGCTGA

Antitoxin


Download         Length: 116 a.a.        Molecular weight: 13206.97 Da        Isoelectric Point: 4.8313

>AT161521 WP_000681609.1 NZ_CP055102:9067-9414 [Shigella flexneri]
MKHLKYKGYLGTVEPDFENNILYGKLAFIRDLVTYEAETLADLEREFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVSHHRAGA

Download         Length: 348 bp

>AT161521 NZ_CP071210:c3786979-3786716 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGGAAAGAATCGCAAAGCTGTTTAAAAACGGCAGAAATCAGGCGGTGAGGTTACCGGTGGAATTTGAATTTGATACGGA
TCGTGTCTATATCCGTCGCGATCGGGAGGGCAACGTGATTTTATCCAAATATCCGGCAAAGCCGGACAGCTGGGCACCGC
TGCTGAGCATTATCGCCCAAACGCCGGTGCCGCAAGAATTTCTTGGCGCGGAAGATCGTCAGCAAGGGATGGCAGACCGC
GATCCGTTCTCAGAGGAAGAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1I9ZJC5

Antitoxin

Source ID Structure
AlphaFold DB A0A1I9ZJ49

References