Detailed information of TA system TA161515

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 1981..2540
• Replicon: plasmid unnamed2
• Accession: NZ_CP055101
• Organism: Shigella flexneri strain SWHIN_107

Toxin (Protein)

• Gene name: relE
• Uniprot ID: U9YB49
• Locus tag: HUZ84_RS24325
• Protein ID: WP_000421259.1
• Coordinates: 2265..2540 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: U9Y625
• Locus tag: HUZ84_RS24320
• Protein ID: WP_001178087.1
• Coordinates: 1981..2265 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ84_RS24315 (46)	46..1077	+	1032	WP_000907852.1	plasmid replication initiator RepA	-
HUZ84_RS24320 (1981)	1981..2265	+	285	WP_001178087.1	ribbon-helix-helix domain-containing protein	Antitoxin
HUZ84_RS24325 (2265)	2265..2540	+	276	WP_000421259.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HUZ84_RS24330 (2646)	2646..2927	+	282	WP_001105066.1	hypothetical protein	-
HUZ84_RS24340 (4455)	4455..4721	-	267	WP_000627832.1	hypothetical protein	-
HUZ84_RS24345 (4721)	4721..5215	-	495	WP_015058884.1	hypothetical protein	-
HUZ84_RS24350 (5271)	5271..5873	-	603	WP_000521606.1	ProQ/FINO family protein	-
HUZ84_RS24355 (6049)	6049..7101	-	1053	WP_000655519.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..88969	88969

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10736.45 Da
• Isoelectric Point: 9.9324

>T161515 WP_000421259.1 NZ_CP055101:2265-2540 [Shigella flexneri]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELTGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T161515 NZ_CP071208:4534605-4534916 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGCATATCATTTCCAGGGAACCGTTCAATAACGCAACGAAGCAATATCCCAATGAAGCCACTGCTCTCGAGGCCATTTA
TAAAGCATTGCGACGGGGCGAGTTCCGCACGCCGGACGAGTTAAAAGCGCTTTTTCCTTCGCTCGATCGTATGAAATACA
AAGAGAAATGGTGGGTCATTGACGTCGGCGGCAACCACCTGCGGATCTTGTTCTTTGCCAGCTTCGAGACACAGAAAGTC
TTCATAAAGCATATCGTTAGCCACGCCGAATACGACAAGCTGATGCAGCACTATCGAAGGAGTTCATCATGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10488.92 Da
• Isoelectric Point: 5.6558

>AT161515 WP_001178087.1 NZ_CP055101:1981-2265 [Shigella flexneri]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWIIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT161515 NZ_CP071208:4534913-4535335 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGATTACAGATGCCATTAAGGCGGCCGATGCGCTACTGCGCGCCGTGCCCTTGTTGCAGGGCAGCCGCGCTCAGCAAGA
CTACCGGGAAGCGCTTGAATTGGTGGAATACCTGCTGGAAAACGACGAACACCATCCGCTGATTGACATGCTGGCAAAAA
AGATCGCCGAATATGAAGACAACAGCGCCGAATTTACCGCATTCAACCAGCGTATCGCCCAGTTACCACAGGGGGTAGCC
GCGCTTCGCGTCCTTATGGAACAGCACCAACTCAGCCAAAGTGATTTTGAACATGAGATCGGTAAAAAGTCGTTGGTTAA
CCAAATCCTGAACGGTAAAAGATCGCTGACCATTCCGCACATTATGGCGCTGGCCAAGCGTTTCAATCTGCCCCCGGCCC
TGTTCCTGCCGGAAGCGGATTAG

Structures

Toxin

• AlphaFold DB: U9YB49

Antitoxin

• AlphaFold DB: U9Y625