Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3510363..3510981 Replicon chromosome
Accession NZ_CP055099
Organism Shigella flexneri strain SWHIN_107

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag HUZ84_RS17010 Protein ID WP_001291435.1
Coordinates 3510763..3510981 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag HUZ84_RS17005 Protein ID WP_000344800.1
Coordinates 3510363..3510737 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ84_RS16995 (3505452) 3505452..3506645 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
HUZ84_RS17000 (3506668) 3506668..3509817 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
HUZ84_RS17005 (3510363) 3510363..3510737 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
HUZ84_RS17010 (3510763) 3510763..3510981 + 219 WP_001291435.1 HHA domain-containing protein Toxin
HUZ84_RS17015 (3511153) 3511153..3511704 + 552 WP_040091271.1 maltose O-acetyltransferase -
HUZ84_RS17020 (3511820) 3511820..3512290 + 471 WP_000136192.1 YlaC family protein -
HUZ84_RS17025 (3512454) 3512454..3514004 + 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
HUZ84_RS17030 (3514046) 3514046..3514399 - 354 WP_000878141.1 DUF1428 family protein -
HUZ84_RS17040 (3514778) 3514778..3515089 + 312 WP_000409911.1 MGMT family protein -
HUZ84_RS17045 (3515120) 3515120..3515692 - 573 WP_000779826.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T161502 WP_001291435.1 NZ_CP055099:3510763-3510981 [Shigella flexneri]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T161502 NZ_CP071206:c3109587-3109189 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGTTCAGTCATATGCTGGACACCAACATTGTTATCTATGTCATCAAGCGCCGGCCGCTGGAAGTGCTGGAGGCATTCAA
TCGCTATGCAGGTAAAATGGTCATTTCGTCGATCACTTATGGCGAATTGGTTCACGGGGTGGAGAAAAGCGCTCGTCCAG
CGGTGAACGCCAGAGTCGTTGAGGATTTTGTCTCCCGATTGGACATTCTCGATTACAGCGCCAAAGCGGCATCGCACTAC
GGCAATATCCGGGCTGCGCTGGAACGACAGGGAACGCCTATTGGCGTAAACGATCTACATATTGCCGGGCATGCGCGCAG
CGAAGGATTGATCCTGGTGACCAATAATCGACGCGAATTTGAGCGCGTTGAGGGTTTGCGGCTGGAAAATTGGTTGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT161502 WP_000344800.1 NZ_CP055099:3510363-3510737 [Shigella flexneri]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT161502 NZ_CP071206:c3109820-3109587 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGACGTTAGGTTCTGTCTTTACTAATAATCGGACACAGGCTGTACGCCTGCCTGCAGAGGCGCGTTTCCCTGATACCGT
TTCTAAAGTGACCGTTCGCGTAGTGGGTAAAGAGCGCATTCTTGCCCCCCTGGAGAATACCTGGGACAGTTTCTTCTGTG
CGGAAAATGAGGTGAGCGATGATTTTCTCAATGAACGTGCCGGACAGCACCAGCGCGAGCGGGAAGCGTTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References