Detailed information of TA system TA161491

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1324080..1324705
• Replicon: chromosome
• Accession: NZ_CP055099
• Organism: Shigella flexneri strain SWHIN_107

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HUZ84_RS06365
• Protein ID: WP_000911330.1
• Coordinates: 1324307..1324705 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: HUZ84_RS06360
• Protein ID: WP_000450524.1
• Coordinates: 1324080..1324307 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ84_RS06335 (1319882)	1319882..1320352	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
HUZ84_RS06340 (1320352)	1320352..1320924	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
HUZ84_RS06345 (1321070)	1321070..1321948	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
HUZ84_RS06350 (1321965)	1321965..1322999	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
HUZ84_RS06355 (1323212)	1323212..1323925	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
HUZ84_RS06360 (1324080)	1324080..1324307	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
HUZ84_RS06365 (1324307)	1324307..1324705	+	399	WP_000911330.1	type II toxin-antitoxin system VapC family toxin	Toxin
HUZ84_RS06370 (1324852)	1324852..1325715	+	864	WP_001267507.1	neutral zinc metallopeptidase	-
HUZ84_RS06375 (1325730)	1325730..1327745	+	2016	WP_032283657.1	tRNA cytosine(34) acetyltransferase TmcA	-
HUZ84_RS06380 (1327819)	1327819..1328517	+	699	WP_000679823.1	esterase	-
HUZ84_RS06385 (1328627)	1328627..1328827	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T161491 WP_000911330.1 NZ_CP055099:1324307-1324705 [Shigella flexneri]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T161491 NZ_CP071204:1601799-1602107 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGCAATTTACCGTATATGACAATACGTATCCGGCATCAGCCTATCCCTACTTACTGGACGTTCAAAGCGATCTTATTGA
TGTCCTCTCGACCCGGTTAATGATCCCTCTTTATGCTTTGGATAATGTCAGGGTTAAAATTTCTGCACGTTTATGCCCGG
AGATTGAGGTCAACGGCGAAAAGTTTCTTGTTATGACGCATGAGATGGCTGCCGTACGTATTTCGCAGATTGGGAAGGCC
GTTGGTAATGTCAATGAGCACAGGAATCAAATTAAAGCCGCCATCGATTTTCTGATTGATGGTTTTTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT161491 WP_000450524.1 NZ_CP055099:1324080-1324307 [Shigella flexneri]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT161491 NZ_CP071204:1601560-1601796 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGGCAAATAACAATGTGACGAAAAAGACAGTCAACGTAACCATCGATCGCGATCTTTTCCAGCGCGCAAAATCCCTGGG
CGTCAATGTGTCATCTGTATTGACGGACGCATTGAGCGTCCGTGTCAGGGACATTGAGATTCAACAGTGGCGCGAGCAAA
ATCGTGCGGCGTTAGAAGAACTTAATCGTATCACTGAAGAAAATGGTTTGTTGTCTGATGAATATCGGACATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33