Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 275522..276322 Replicon chromosome
Accession NZ_CP055099
Organism Shigella flexneri strain SWHIN_107

Toxin (Protein)


Gene name ataT Uniprot ID F4NNI0
Locus tag HUZ84_RS01285 Protein ID WP_000342449.1
Coordinates 275795..276322 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag HUZ84_RS01280 Protein ID WP_001277108.1
Coordinates 275522..275788 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ84_RS01260 (271180) 271180..271848 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
HUZ84_RS01265 (271841) 271841..272899 + 1059 WP_001042003.1 permease-like cell division protein FtsX -
HUZ84_RS01270 (273144) 273144..273998 + 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
HUZ84_RS01275 (274269) 274269..275372 + 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
HUZ84_RS01280 (275522) 275522..275788 + 267 WP_001277108.1 type II toxin-antitoxin system antitoxin AtaR Antitoxin
HUZ84_RS01285 (275795) 275795..276322 + 528 WP_000342449.1 type II toxin-antitoxin system toxin acetyltransferase AtaT Toxin
HUZ84_RS01290 (276319) 276319..276702 - 384 WP_000778785.1 aspartate 1-decarboxylase autocleavage activator PanM -
HUZ84_RS01295 (277126) 277126..278235 + 1110 WP_000827696.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
HUZ84_RS01300 (278283) 278283..279209 + 927 WP_001295111.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
HUZ84_RS01305 (279206) 279206..280483 + 1278 WP_000803784.1 branched chain amino acid ABC transporter permease LivM -
HUZ84_RS01310 (280480) 280480..281247 + 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19691.70 Da        Isoelectric Point: 7.7457

>T161487 WP_000342449.1 NZ_CP055099:275795-276322 [Shigella flexneri]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

>T161487 NZ_CP071202:c4454608-4454354 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGAACAAACGACACCAAAAAACGCTGTCAGATGTGTTTGCCCGGCCTGTCAACGGTTCTATAAAGTGGTCTGATATTGA
GGCGCTTTTTACCGCATTAGGGGCGGAGATTCACGAAAGGGAAGGTTCTAGGATCGCGGTGCTGTTGAAAGGTGAAAAAA
GAGTCTTTCACCGGCCACACCCCAGACCTACCACTGACAAGGGGGCGGTTAACTCCATTCGGATCTGGTTGGATAGCTTA
GGAATAAAACCATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT161487 WP_001277108.1 NZ_CP055099:275522-275788 [Shigella flexneri]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT161487 NZ_CP071202:c4454354-4454013 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGAATAACACACTGAAAATTGACGGCCATACGGCCGTCATCAACTTCGACCCTGAAATTGAAATGTTCCGGGGCGAGTT
TGTCGGGCTGAATGGCGGCGCCGACTTCTACGCCTACAGCGTGGACGAGCTGAAGAAAGAAGGCGCGATCTCACTCGCGG
TCTTTCTCGATGAGTGCCATAAAGACGGCATCGAGCCTTACAAGTCGTACAGCGGCAAAGTAACCACCCGCCTGTCGCCG
GAACGCCATCAGGCGTTAGCCATTGCCGCACAGGCCACCGGGCAGTCGATTAATGAACTGCTGAATGAAGGTGTTGATCT
GGTTATCGAAAAGCATTCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLZ4

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References