Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 5864..6389 Replicon plasmid unnamed1
Accession NZ_CP055097
Organism Klebsiella pneumoniae strain SWHIN_108

Toxin (Protein)


Gene name ccdB Uniprot ID D4HQE7
Locus tag HUZ85_RS25475 Protein ID WP_013023785.1
Coordinates 5864..6169 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID W8V2V6
Locus tag HUZ85_RS25480 Protein ID WP_001568025.1
Coordinates 6171..6389 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ85_RS25445 (HUZ85_25385) 1567..2193 + 627 WP_004197649.1 ParA family plasmid-partitioning AAA ATPase -
HUZ85_RS25450 2190..2492 + 303 WP_004197636.1 hypothetical protein -
HUZ85_RS25455 (HUZ85_25395) 2940..3734 - 795 WP_004197635.1 site-specific integrase -
HUZ85_RS25460 (HUZ85_25400) 3932..4948 - 1017 WP_017899884.1 hypothetical protein -
HUZ85_RS25465 (HUZ85_25405) 4959..5273 - 315 WP_053389906.1 hypothetical protein -
HUZ85_RS25470 (HUZ85_25410) 5300..5695 - 396 WP_017899885.1 hypothetical protein -
HUZ85_RS25475 (HUZ85_25415) 5864..6169 - 306 WP_013023785.1 type II toxin-antitoxin system toxin CcdB Toxin
HUZ85_RS25480 (HUZ85_25420) 6171..6389 - 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
HUZ85_RS25485 (HUZ85_25425) 7207..7497 - 291 WP_013023783.1 hypothetical protein -
HUZ85_RS25490 (HUZ85_25430) 7494..8621 - 1128 WP_013023782.1 DUF4238 domain-containing protein -
HUZ85_RS25495 (HUZ85_25435) 8655..10247 - 1593 WP_015344964.1 hypothetical protein -
HUZ85_RS25500 (HUZ85_25440) 10454..11233 - 780 WP_013023780.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid sul2 / aph(3'')-Ib / aph(6)-Id / aph(3')-Ia / aac(3)-IId / aac(6')-Ib-cr / ARR-3 / dfrA27 / aadA16 / qacE / sul1 / qnrB91 / mph(A) / tet(A) / floR / blaTEM-1B / blaCTX-M-3 / qnrS1 - 1..91928 91928


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11615.30 Da        Isoelectric Point: 6.4672

>T161485 WP_013023785.1 NZ_CP055097:c6169-5864 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T161485 NZ_CP071202:c3786732-3786343 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGTACATGTTTGATACCAACACCGTCAGCCACTTGTTTCGGCAGCATCCGCAGGTGCTTAACGTCATGGAAAAGCTCCC
GCCTTCTGCCGTATGTATTTCCAGCGTCACGGAGGCGGAACTGCTTTACGGTGTGGCTAAACGGCGGAACAAGGCGTTGC
AAAGCATGGTCGAGGCCTTTCTTGCTGCAGTGACGGTCTATGCATGGGATAGCGAGGCCGCCCGTTGCTACGGTGAAATG
CGGGCAACCATGGAGAGGAAGGGCAAAATCATGGGCGCGATCGATCAACTGATCGCCGCTCACGCGCAGAGCCGTGGCGC
GACGCTGGTGACCAACGATCGCGCATTCGCCATGGTGCCCGGGTTGGCGGTTGAAGACTGGACGCGCTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8392.34 Da        Isoelectric Point: 4.7405

>AT161485 WP_001568025.1 NZ_CP055097:c6389-6171 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT161485 NZ_CP071202:c3786999-3786736 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGGAAAGAATCGCAAAGCTGTTTAAAAACGGCAGAAATCAGGCGGTGAGGTTACCGGTGGAATTTGAATTTGATACGGA
TCGTGTCTATATCCGTCGCGATCGGGAGGGCAACGTGATTTTATCCAAATATCCGGCAAAGCCGGACAGCTGGGCACCGC
TGCTGAGCATTATCGCCCAAACGCCGGTGCCGCAAGAATTTCTTGGCGCGGAAGATCGTCAGCAAGGGATGGCAGACCGC
GATCCGTTCTCAGAGGAAGAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J4ZZW6

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZZP8

References