Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5150270..5150895 Replicon chromosome
Accession NZ_CP055096
Organism Klebsiella pneumoniae strain SWHIN_108

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag HUZ85_RS25075 Protein ID WP_002882817.1
Coordinates 5150270..5150653 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag HUZ85_RS25080 Protein ID WP_004150355.1
Coordinates 5150653..5150895 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ85_RS25060 (5147636) 5147636..5148538 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
HUZ85_RS25065 (5148535) 5148535..5149170 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
HUZ85_RS25070 (5149167) 5149167..5150096 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
HUZ85_RS25075 (5150270) 5150270..5150653 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
HUZ85_RS25080 (5150653) 5150653..5150895 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
HUZ85_RS25085 (5151100) 5151100..5152017 + 918 WP_032418237.1 alpha/beta hydrolase -
HUZ85_RS25090 (5152031) 5152031..5152972 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
HUZ85_RS25095 (5153017) 5153017..5153454 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
HUZ85_RS25100 (5153451) 5153451..5154311 - 861 WP_004146232.1 virulence factor BrkB family protein -
HUZ85_RS25105 (5154305) 5154305..5154904 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T161484 WP_002882817.1 NZ_CP055096:c5150653-5150270 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T161484 NZ_CP071202:c3109587-3109189 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGTTCAGTCATATGCTGGACACCAACATTGTTATCTATGTCATCAAGCGCCGGCCGCTGGAAGTGCTGGAGGCATTCAA
TCGCTATGCAGGTAAAATGGTCATTTCGTCGATCACTTATGGCGAATTGGTTCACGGGGTGGAGAAAAGCGCTCGTCCAG
CGGTGAACGCCAGAGTCGTTGAGGATTTTGTCTCCCGATTGGACATTCTCGATTACAGCGCCAAAGCGGCATCGCACTAC
GGCAATATCCGGGCTGCGCTGGAACGACAGGGAACGCCTATTGGCGTAAACGATCTACATATTGCCGGGCATGCGCGCAG
CGAAGGATTGATCCTGGTGACCAATAATCGACGCGAATTTGAGCGCGTTGAGGGTTTGCGGCTGGAAAATTGGTTGTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT161484 WP_004150355.1 NZ_CP055096:c5150895-5150653 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT161484 NZ_CP071202:c3109820-3109587 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGACGTTAGGTTCTGTCTTTACTAATAATCGGACACAGGCTGTACGCCTGCCTGCAGAGGCGCGTTTCCCTGATACCGT
TTCTAAAGTGACCGTTCGCGTAGTGGGTAAAGAGCGCATTCTTGCCCCCCTGGAGAATACCTGGGACAGTTTCTTCTGTG
CGGAAAATGAGGTGAGCGATGATTTTCTCAATGAACGTGCCGGACAGCACCAGCGCGAGCGGGAAGCGTTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References