Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3943945..3944564 Replicon chromosome
Accession NZ_CP055096
Organism Klebsiella pneumoniae strain SWHIN_108

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag HUZ85_RS19295 Protein ID WP_002892050.1
Coordinates 3944346..3944564 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag HUZ85_RS19290 Protein ID WP_002892066.1
Coordinates 3943945..3944319 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ85_RS19280 (3939097) 3939097..3940290 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
HUZ85_RS19285 (3940313) 3940313..3943459 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
HUZ85_RS19290 (3943945) 3943945..3944319 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
HUZ85_RS19295 (3944346) 3944346..3944564 + 219 WP_002892050.1 HHA domain-containing protein Toxin
HUZ85_RS19300 (3944723) 3944723..3945289 + 567 WP_002892030.1 maltose O-acetyltransferase -
HUZ85_RS19305 (3945261) 3945261..3945401 - 141 WP_004147370.1 hypothetical protein -
HUZ85_RS19310 (3945422) 3945422..3945892 + 471 WP_002892026.1 YlaC family protein -
HUZ85_RS19315 (3945867) 3945867..3947318 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
HUZ85_RS19320 (3947419) 3947419..3948117 + 699 WP_002892021.1 GNAT family protein -
HUZ85_RS19325 (3948114) 3948114..3948254 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
HUZ85_RS19330 (3948254) 3948254..3948517 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T161481 WP_002892050.1 NZ_CP055096:3944346-3944564 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T161481 NZ_CP071202:c1108033-1107830 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGACTAAAACCGACTATCTGATGCGTTTACGTAAATGCACCACGATTGATACTCTCGAACGTGTTATTGAGAAAAATAA
GTACGAGCTTTCCGATGATGAACTGGAGCTGTTTTACTCAGCGGCCGATCACCGTCTGGCAGAGCTCACCATGAACAAAC
TGTATGACAAAATCCCCACCTCCGTTTGGAAATATGTCAGATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT161481 WP_002892066.1 NZ_CP055096:3943945-3944319 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT161481 NZ_CP071202:c1108451-1108083 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGGATGAGTATTCGCCTAAGCGGCACGACATTGCTCAGCTCAAATTCTTGTGCGAAAATTTGTATGATGAAGGTATCGC
TACCCTGGGAGACAGTCATCATGGGTGGGTCAACGATCCGACGTCCTCCGTCAACCTGCAGCTTAATGAGTTGATCGAAC
ATATCGCTTCGTTTGTGATGAGTTATAAAATTAAATACATGGACGAATCTGACCTGTCGGAGTTAGTCGAAGAATATCTC
GACGATACCTATACGCTGTTCAGCAGCTATGGTATCAATGATTCCGACCTGCGCCGCTGGCAAAAAACCAAGGCGAGATT
ATTCAGAATGTTCTCAGGAGAGGACATCTGTACGACAATGAAAACTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References