Detailed information of TA system TA161470

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 1761938..1762528
• Replicon: chromosome
• Accession: NZ_CP055096
• Organism: Klebsiella pneumoniae strain SWHIN_108

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: HUZ85_RS08515
• Protein ID: WP_023341911.1
• Coordinates: 1762196..1762528 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: A0A3S7DDD0
• Locus tag: HUZ85_RS08510
• Protein ID: WP_000288812.1
• Coordinates: 1761938..1762195 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ85_RS08485 (1757848)	1757848..1757922	+	75	Protein_1663	type II toxin-antitoxin system PemK/MazF family toxin	-
HUZ85_RS08490 (1758273)	1758273..1760021	+	1749	WP_032418698.1	hypothetical protein	-
HUZ85_RS08495 (1760075)	1760075..1760656	+	582	WP_099184326.1	hypothetical protein	-
HUZ85_RS08500 (1760939)	1760939..1761400	+	462	WP_004213450.1	hypothetical protein	-
HUZ85_RS08505 (1761397)	1761397..1761603	+	207	WP_020805021.1	helix-turn-helix transcriptional regulator	-
HUZ85_RS08510 (1761938)	1761938..1762195	+	258	WP_000288812.1	antitoxin	Antitoxin
HUZ85_RS08515 (1762196)	1762196..1762528	+	333	WP_023341911.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
HUZ85_RS08525 (1762850)	1762850..1764286	+	1437	WP_004151469.1	EmmdR/YeeO family multidrug/toxin efflux MATE transporter	-
HUZ85_RS08535 (1764652)	1764652..1766106	-	1455	WP_004148975.1	AMP nucleosidase	-
HUZ85_RS08540 (1766236)	1766236..1766481	-	246	WP_004141189.1	signal transduction protein PmrD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-108)

Antitoxin

• Antitoxin PemI-like (4-84)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11927.87 Da
• Isoelectric Point: 10.4722

>T161470 WP_023341911.1 NZ_CP055096:1762196-1762528 [Klebsiella pneumoniae]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARKGKRLERIPDVVVNEVLARLDAMLS

Nucleotide

• Length: 333 bp

>T161470 NZ_CP071198:4534603-4534914 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGCATATCATTTCCAGGGAACCGTTCAATAACGCAACGAAGCAATATCCCAATGAAGCCACTGCTCTCGAGGCCATTTA
TAAAGCATTGCGACGGGGCGAGTTCCGCACGCCGGACGAGTTAAAAGCGCTTTTTCCTTCGCTCGATCGTATGAAATACA
AAGAGAAATGGTGGGTCATTGACGTCGGCGGCAACCACCTGCGGATCTTGTTCTTTGCCAGCTTCGAGACACAGAAAGTC
TTCATAAAGCATATCGTTAGCCACGCCGAATACGACAAGCTGATGCAGCACTATCGAAGGAGTTCATCATGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9442.92 Da
• Isoelectric Point: 4.3566

>AT161470 WP_000288812.1 NZ_CP055096:1761938-1762195 [Klebsiella pneumoniae]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Nucleotide

• Length: 258 bp

>AT161470 NZ_CP071198:4534911-4535333 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGATTACAGATGCCATTAAGGCGGCCGATGCGCTACTGCGCGCCGTGCCCTTGTTGCAGGGCAGCCGCGCTCAGCAAGA
CTACCGGGAAGCGCTTGAATTGGTGGAATACCTGCTGGAAAACGACGAACACCATCCGCTGATTGACATGCTGGCAAAAA
AGATCGCCGAATATGAAGACAACAGCGCCGAATTTACCGCATTCAACCAGCGTATCGCCCAGTTACCACAGGGGGTAGCC
GCGCTTCGCGTCCTTATGGAACAGCACCAACTCAGCCAAAGTGATTTTGAACATGAGATCGGTAAAAAGTCGTTGGTTAA
CCAAATCCTGAACGGTAAAAGATCGCTGACCATTCCGCACATTATGGCGCTGGCCAAGCGTTTCAATCTGCCCCCGGCCC
TGTTCCTGCCGGAAGCGGATTAG

Structures

Antitoxin

• AlphaFold DB: A0A3S7DDD0