Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3399366..3399984 Replicon chromosome
Accession NZ_CP055092
Organism Shigella flexneri strain SWHIN_110

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag HUZ87_RS16465 Protein ID WP_001291435.1
Coordinates 3399766..3399984 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag HUZ87_RS16460 Protein ID WP_000344800.1
Coordinates 3399366..3399740 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ87_RS16450 (3394455) 3394455..3395648 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
HUZ87_RS16455 (3395671) 3395671..3398820 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
HUZ87_RS16460 (3399366) 3399366..3399740 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
HUZ87_RS16465 (3399766) 3399766..3399984 + 219 WP_001291435.1 HHA domain-containing protein Toxin
HUZ87_RS16470 (3400156) 3400156..3400707 + 552 WP_000102564.1 maltose O-acetyltransferase -
HUZ87_RS16475 (3400823) 3400823..3401293 + 471 WP_000136192.1 YlaC family protein -
HUZ87_RS16480 (3401457) 3401457..3403007 + 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
HUZ87_RS16485 (3403049) 3403049..3403402 - 354 WP_000878140.1 DUF1428 domain-containing protein -
HUZ87_RS16495 (3403781) 3403781..3404092 + 312 WP_000409911.1 MGMT family protein -
HUZ87_RS16500 (3404123) 3404123..3404695 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T161459 WP_001291435.1 NZ_CP055092:3399766-3399984 [Shigella flexneri]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T161459 NZ_CP071196:c4164440-4164018 [Serratia marcescens subsp. marcescens ATCC 13880]
GTGGCCCAGTGGCGATGTGATATCCGCATTTCCTGGCGCACCCAGCTGTTTTCACTGTTGACCCACGGCGTTCTGATCCT
GCTGATCCTGATTTCGCCCTGGCCGGAGGGCTTTGGCCCGCTCTGGCTGGTGCTGCTGACGCTGGTGGTGTTTCAGTGCA
TTCGCAGCCAGAAGCGCATCGCCGCGGTGCAGGGTGAGCTGCGGCTGCTGGCGGACAGGCGTTTCAGCTGGCACGGCCGC
GAGTGGCGGCTGGCGAAAAAACCGTGGATGCCGGGTTACGGCATGCTGTTGACGCTGCAGCCGATGGAGGGCAAAAAGCG
CCGCCGGCTGTGGCTGGCTTCCGACTGCATGAGCAAAGAGGAGTGGCGCCACCTGCGGCAGCTGTTGCTGCATCCGCCGG
CGGGTAACGGCGAGGAACCCTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT161459 WP_000344800.1 NZ_CP055092:3399366-3399740 [Shigella flexneri]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT161459 NZ_CP071196:c4164687-4164421 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGGATATTAACAACAAAGCACGTATTCACTGGGCTTGCCGTCGTGGCATGCGCGAGCTGGACATCTCCATCATGCCGTT
CTTCGAATACGAATACGACAGCCTGAATGACGCGGACAAGGCGCTGTTTATCCGCCTGCTGGAGTGTGACGATCCCGATT
TGTTCAACTGGCTGATGAATCATGGCGCGCCGCAGGATGGCGAGCTGCAGAGAATGGTGACCCTGATCCAAACGCGAAAT
AAAGACCGTGGCCCAGTGGCGATGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References