Detailed information of TA system TA161458

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2368228..2368866
• Replicon: chromosome
• Accession: NZ_CP055092
• Organism: Shigella flexneri strain SWHIN_110

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0A6TXU8
• Locus tag: HUZ87_RS11450
• Protein ID: WP_001447010.1
• Coordinates: 2368690..2368866 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: HUZ87_RS11445
• Protein ID: WP_001270286.1
• Coordinates: 2368228..2368644 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ87_RS11425 (2363380)	2363380..2364321	-	942	WP_001251304.1	ABC transporter permease	-
HUZ87_RS11430 (2364322)	2364322..2365335	-	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
HUZ87_RS11435 (2365353)	2365353..2366498	-	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
HUZ87_RS11440 (2366743)	2366743..2368149	-	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
HUZ87_RS11445 (2368228)	2368228..2368644	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
HUZ87_RS11450 (2368690)	2368690..2368866	-	177	WP_001447010.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
HUZ87_RS11455 (2369088)	2369088..2369318	+	231	WP_000494244.1	YncJ family protein	-
HUZ87_RS11460 (2369410)	2369410..2371371	-	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
HUZ87_RS11465 (2371444)	2371444..2371980	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
HUZ87_RS11470 (2372072)	2372072..2373247	+	1176	WP_001236265.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2373287..2374435	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6779.86 Da
• Isoelectric Point: 11.2298

>T161458 WP_001447010.1 NZ_CP055092:c2368866-2368690 [Shigella flexneri]
VKQSEFRRWLESQGVDVANSSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T161458 NZ_CP071196:c3786712-3786323 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGTACATGTTTGATACCAACACCGTCAGCCACTTGTTTCGGCAGCATCCGCAGGTGCTTAACGTCATGGAAAAGCTCCC
GCCTTCTGCCGTATGTATTTCCAGCGTCACGGAGGCGGAACTGCTTTACGGTGTGGCTAAACGGCGGAACAAGGCGTTGC
AAAGCATGGTCGAGGCCTTTCTTGCTGCAGTGACGGTCTATGCATGGGATAGCGAGGCCGCCCGTTGCTACGGTGAAATG
CGGGCAACCATGGAGAGGAAGGGCAAAATCATGGGCGCGATCGATCAACTGATCGCCGCTCACGCGCAGAGCCGTGGCGC
GACGCTGGTGACCAACGATCGCGCATTCGCCATGGTGCCCGGGTTGGCGGTTGAAGACTGGACGCGCTGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT161458 WP_001270286.1 NZ_CP055092:c2368644-2368228 [Shigella flexneri]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT161458 NZ_CP071196:c3786979-3786716 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGGAAAGAATCGCAAAGCTGTTTAAAAACGGCAGAAATCAGGCGGTGAGGTTACCGGTGGAATTTGAATTTGATACGGA
TCGTGTCTATATCCGTCGCGATCGGGAGGGCAACGTGATTTTATCCAAATATCCGGCAAAGCCGGACAGCTGGGCACCGC
TGCTGAGCATTATCGCCCAAACGCCGGTGCCGCAAGAATTTCTTGGCGCGGAAGATCGTCAGCAAGGGATGGCAGACCGC
GATCCGTTCTCAGAGGAAGAGTAG

Structures

Toxin

• AlphaFold DB: A0A0A6TXU8