Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 1322580..1323205 Replicon chromosome
Accession NZ_CP055092
Organism Shigella flexneri strain SWHIN_110

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag HUZ87_RS06410 Protein ID WP_000911329.1
Coordinates 1322807..1323205 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag HUZ87_RS06405 Protein ID WP_000450524.1
Coordinates 1322580..1322807 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ87_RS06380 (1318381) 1318381..1318851 - 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -
HUZ87_RS06385 (1318851) 1318851..1319423 - 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
HUZ87_RS06390 (1319569) 1319569..1320447 + 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
HUZ87_RS06395 (1320464) 1320464..1321498 + 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
HUZ87_RS06400 (1321711) 1321711..1322424 + 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
HUZ87_RS06405 (1322580) 1322580..1322807 + 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
HUZ87_RS06410 (1322807) 1322807..1323205 + 399 WP_000911329.1 type II toxin-antitoxin system VapC family toxin Toxin
HUZ87_RS06415 (1323352) 1323352..1324215 + 864 WP_001267507.1 neutral zinc metallopeptidase -
HUZ87_RS06420 (1324230) 1324230..1326245 + 2016 WP_000829332.1 tRNA cytosine(34) acetyltransferase TmcA -
HUZ87_RS06425 (1326319) 1326319..1327017 + 699 WP_000679812.1 esterase -
HUZ87_RS06430 (1327127) 1327127..1327327 - 201 WP_000383836.1 YpfN family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T161446 WP_000911329.1 NZ_CP055092:1322807-1323205 [Shigella flexneri]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T161446 NZ_CP071194:1601799-1602107 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGCAATTTACCGTATATGACAATACGTATCCGGCATCAGCCTATCCCTACTTACTGGACGTTCAAAGCGATCTTATTGA
TGTCCTCTCGACCCGGTTAATGATCCCTCTTTATGCTTTGGATAATGTCAGGGTTAAAATTTCTGCACGTTTATGCCCGG
AGATTGAGGTCAACGGCGAAAAGTTTCTTGTTATGACGCATGAGATGGCTGCCGTACGTATTTCGCAGATTGGGAAGGCC
GTTGGTAATGTCAATGAGCACAGGAATCAAATTAAAGCCGCCATCGATTTTCTGATTGATGGTTTTTGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT161446 WP_000450524.1 NZ_CP055092:1322580-1322807 [Shigella flexneri]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT161446 NZ_CP071194:1601560-1601796 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGGCAAATAACAATGTGACGAAAAAGACAGTCAACGTAACCATCGATCGCGATCTTTTCCAGCGCGCAAAATCCCTGGG
CGTCAATGTGTCATCTGTATTGACGGACGCATTGAGCGTCCGTGTCAGGGACATTGAGATTCAACAGTGGCGCGAGCAAA
ATCGTGCGGCGTTAGAAGAACTTAATCGTATCACTGAAGAAAATGGTTTGTTGTCTGATGAATATCGGACATTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References