Detailed information of TA system TA161421

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 5306..5949
• Replicon: plasmid unnamed1
• Accession: NZ_CP055088
• Organism: Klebsiella pneumoniae strain SWHIN_114

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q84A06
• Locus tag: HUZ89_RS26835
• Protein ID: WP_000754566.1
• Coordinates: 5306..5722 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q84A07
• Locus tag: HUZ89_RS26840
• Protein ID: WP_001261276.1
• Coordinates: 5719..5949 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ89_RS26810 (HUZ89_26750)	708..1412	-	705	WP_001067834.1	IS6-like element IS26 family transposase	-
HUZ89_RS26815 (HUZ89_26755)	1480..2001	+	522	Protein_2	Tn3 family transposase	-
HUZ89_RS26820 (HUZ89_26760)	2164..2247	-	84	Protein_3	SOS response-associated peptidase	-
HUZ89_RS26825 (HUZ89_26765)	2888..3964	-	1077	WP_000227969.1	IS110 family transposase	-
HUZ89_RS26830 (HUZ89_26770)	4246..5102	-	857	Protein_5	IS3-like element ISEc15 family transposase	-
HUZ89_RS26835 (HUZ89_26775)	5306..5722	-	417	WP_000754566.1	type II toxin-antitoxin system VapC family toxin	Toxin
HUZ89_RS26840 (HUZ89_26780)	5719..5949	-	231	WP_001261276.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
HUZ89_RS26845 (HUZ89_26785)	6523..6873	+	351	WP_000493378.1	hypothetical protein	-
HUZ89_RS26850 (HUZ89_26790)	6924..7667	+	744	WP_000129823.1	hypothetical protein	-
HUZ89_RS26855 (HUZ89_26795)	7664..8440	+	777	WP_000015958.1	site-specific integrase	-
HUZ89_RS26860 (HUZ89_26800)	8498..8755	-	258	WP_000764642.1	hypothetical protein	-
HUZ89_RS26865 (HUZ89_26805)	8884..8988	-	105	WP_032409716.1	hypothetical protein	-
HUZ89_RS26870 (HUZ89_26810)	9518..10384	+	867	WP_004118283.1	replication initiation protein	-
HUZ89_RS26875 (HUZ89_26815)	10561..10830	-	270	WP_000339857.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	tet(A) / dfrA12 / aadA2 / qacE / sul1 / mph(A)	-	1..42893	42893

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15006.52 Da
• Isoelectric Point: 9.2957

>T161421 WP_000754566.1 NZ_CP055088:c5722-5306 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK

Nucleotide

• Length: 417 bp

>T161421 NZ_CP071188:c3109575-3109177 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGTTCAGTCATATGCTGGACACCAACATTGTTATCTATGTCATCAAGCGCCGGCCGCTGGAAGTGCTGGAGGCATTCAA
TCGCTATGCAGGTAAAATGGTCATTTCGTCGATCACTTATGGCGAATTGGTTCACGGGGTGGAGAAAAGCGCTCGTCCAG
CGGTGAACGCCAGAGTCGTTGAGGATTTTGTCTCCCGATTGGACATTCTCGATTACAGCGCCAAAGCGGCATCGCACTAC
GGCAATATCCGGGCTGCGCTGGAACGACAGGGAACGCCTATTGGCGTAAACGATCTACATATTGCCGGGCATGCGCGCAG
CGAAGGATTGATCCTGGTGACCAATAATCGACGCGAATTTGAGCGCGTTGAGGGTTTGCGGCTGGAAAATTGGTTGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8721.74 Da
• Isoelectric Point: 4.3533

>AT161421 WP_001261276.1 NZ_CP055088:c5949-5719 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT161421 NZ_CP071188:c3109808-3109575 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGACGTTAGGTTCTGTCTTTACTAATAATCGGACACAGGCTGTACGCCTGCCTGCAGAGGCGCGTTTCCCTGATACCGT
TTCTAAAGTGACCGTTCGCGTAGTGGGTAAAGAGCGCATTCTTGCCCCCCTGGAGAATACCTGGGACAGTTTCTTCTGTG
CGGAAAATGAGGTGAGCGATGATTTTCTCAATGAACGTGCCGGACAGCACCAGCGCGAGCGGGAAGCGTTTTGA

Structures

Toxin

• AlphaFold DB: A0A387K1G3

Antitoxin

• AlphaFold DB: A0A387K023