Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5381158..5381783 Replicon chromosome
Accession NZ_CP055087
Organism Klebsiella pneumoniae strain SWHIN_114

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag HUZ89_RS26440 Protein ID WP_002882817.1
Coordinates 5381158..5381541 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag HUZ89_RS26445 Protein ID WP_004150355.1
Coordinates 5381541..5381783 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ89_RS26425 (HUZ89_26365) 5378524..5379426 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
HUZ89_RS26430 (HUZ89_26370) 5379423..5380058 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
HUZ89_RS26435 (HUZ89_26375) 5380055..5380984 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
HUZ89_RS26440 (HUZ89_26380) 5381158..5381541 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
HUZ89_RS26445 (HUZ89_26385) 5381541..5381783 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
HUZ89_RS26450 (HUZ89_26390) 5381988..5382905 + 918 WP_002882812.1 alpha/beta hydrolase -
HUZ89_RS26455 (HUZ89_26395) 5382919..5383860 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
HUZ89_RS26460 (HUZ89_26400) 5383905..5384342 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
HUZ89_RS26465 (HUZ89_26405) 5384339..5385199 - 861 WP_002882807.1 virulence factor BrkB family protein -
HUZ89_RS26470 (HUZ89_26410) 5385193..5385792 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T161420 WP_002882817.1 NZ_CP055087:c5381541-5381158 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T161420 NZ_CP071188:2039708-2039804 [Serratia marcescens subsp. marcescens ATCC 13880]
AACAAGCCCTGCATTAAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTAGACCGAATATAGGAATC
GTATTCGGTCTTTTTTT

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT161420 WP_004150355.1 NZ_CP055087:c5381783-5381541 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT161420 NZ_CP071188:c2039804-2039664 [Serratia marcescens subsp. marcescens ATCC 13880]
AAAAAAAGACCGAATACGATTCCTATATTCGGTCTAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCAT
TTAATGCAGGGCTTGTTCAGCCGTGCACTTTAAGAGTAGCCTACCGCGCCAGTTTTGCCAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References