Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4158840..4159459 Replicon chromosome
Accession NZ_CP055087
Organism Klebsiella pneumoniae strain SWHIN_114

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag HUZ89_RS20610 Protein ID WP_002892050.1
Coordinates 4159241..4159459 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag HUZ89_RS20605 Protein ID WP_002892066.1
Coordinates 4158840..4159214 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ89_RS20595 (HUZ89_20540) 4153992..4155185 + 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
HUZ89_RS20600 (HUZ89_20545) 4155208..4158354 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
HUZ89_RS20605 (HUZ89_20550) 4158840..4159214 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
HUZ89_RS20610 (HUZ89_20555) 4159241..4159459 + 219 WP_002892050.1 HHA domain-containing protein Toxin
HUZ89_RS20615 (HUZ89_20560) 4159618..4160184 + 567 WP_002892030.1 maltose O-acetyltransferase -
HUZ89_RS20620 (HUZ89_20565) 4160156..4160296 - 141 WP_004147370.1 hypothetical protein -
HUZ89_RS20625 (HUZ89_20570) 4160317..4160787 + 471 WP_002892026.1 YlaC family protein -
HUZ89_RS20630 (HUZ89_20575) 4160762..4162213 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
HUZ89_RS20635 (HUZ89_20580) 4162314..4163012 + 699 WP_002892021.1 GNAT family protein -
HUZ89_RS20640 (HUZ89_20585) 4163009..4163149 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
HUZ89_RS20645 (HUZ89_20590) 4163149..4163412 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T161415 WP_002892050.1 NZ_CP055087:4159241-4159459 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T161415 NZ_CP071181:c33304-33029 [Listeria innocua]
ATGAGTAAACATTTTCATGTGAGATTTGAAAAAGAAGCTCAAAAAGTATTAAAAAAGATGGATAGATTTCAAGCAAAATT
GATATTATCCTGGATTGAAAAACATATTGAAGGTACTGATGATCCAAGACAACATGGTAAAAGTCTTGTTGGTAACCGGA
GTGGACAATGGCGATATAGGGTTGGAGATTATCGCTTAATTGCAGAGATTCAAGATAATGAAGTGATAGTGTTAATTTTA
AACATCGGACACAGGAGAGATATTTACGATAAATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT161415 WP_002892066.1 NZ_CP055087:4158840-4159214 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT161415 NZ_CP071181:c33522-33301 [Listeria innocua]
ATGACTACCATTACATTTCGAGTATCTGATAAAGAAAAAGCGTTTATTCAAGAAATGGCTAAGCTTAACGGTATCTCTAT
TTCAGAACTTAGTCGTAAACAGATTATAGAAGGTTTAGAAGATCAAATTGATTTACAATCGTATGAGCGTGCTATAAAGG
CGCATCAAGTGAAAGATGAAAGTATTTCTTTTGATGAAATGAAAAAGGAACTTGAATTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References