Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 103433..104076 Replicon plasmid unnamed1
Accession NZ_CP055076
Organism Shigella flexneri strain SWHIN_116

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag HUZ90_RS23400 Protein ID WP_001034044.1
Coordinates 103660..104076 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag HUZ90_RS23395 Protein ID WP_001261286.1
Coordinates 103433..103663 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ90_RS23380 (HUZ90_23150) 98570..98800 + 231 WP_001261278.1 type II toxin-antitoxin system VapB family antitoxin -
HUZ90_RS23385 (HUZ90_23155) 98797..99213 + 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin -
HUZ90_RS23390 (HUZ90_23160) 99258..103052 - 3795 WP_001144732.1 hypothetical protein -
HUZ90_RS23395 (HUZ90_23165) 103433..103663 + 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
HUZ90_RS23400 (HUZ90_23170) 103660..104076 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
HUZ90_RS23405 (HUZ90_23175) 104151..105716 + 1566 WP_001128474.1 AAA family ATPase -
HUZ90_RS23410 (HUZ90_23180) 105701..106723 + 1023 WP_000361402.1 helicase UvrD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid tet(B) / catA1 / mph(A) / sul1 / qacE / aadA5 / blaTEM-1B / sul2 / aph(3'')-Ib / aph(6)-Id / aph(3')-Ia / aac(3)-IId / sitABCD iutA / iucD / iucC / iucB / iucA 1..125787 125787
- flank IS/Tn - - 106977..107480 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T161395 WP_001034044.1 NZ_CP055076:103660-104076 [Shigella flexneri]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

>T161395 NZ_CP071155:882319-882666 [Listeria innocua]
CTGATGGTGAAGCGTGGTGACGTTTACTACGCGGACCTTTCCCCCGTGGTCGGAAGCGAGCAAGGGGGAATAAGGCCTGT
TCTCATCATTCAAAATGATATTGGTAATAGATTCAGTCCAACTGTGATCGTGGCAGCAATAACTGCAAAAATTCAAAAAG
CGAAGTTGCCAACACATGTGGAAGCTACTCGTAAAGACGGCTTTGAAAGAGATTCTGTCATTCTATTAGAACAAATTAGA
ACGATAGATAAACAACGCCTGACAGATAAGATTACACATTTGGACGAGGAACTAATGGCCAAGGTAAATCAGGCACTTGA
AGTCAGTCTAGGAGTAGTAGAATTCTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT161395 WP_001261286.1 NZ_CP055076:103433-103663 [Shigella flexneri]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp

>AT161395 NZ_CP071155:882037-882315 [Listeria innocua]
GTGTTAGAGAAAGAAAAACGGATGATAATATCCGTAGAACTGACACAGGAAATGGTACAAGAACTCGACGTAGTTGTAGA
AAAAGAAAAAATGGGGCGGAGTGAAGTTATAATGGAAGCAACGCAACAATTTTTACAGGAGAAAAGAGCTCGTGAATTAA
GAGACGAGATGGAACGCGGCTATGCAGAAATGGCGACGATTAATTTCGCTATCGCGTGCGAGTGTACTCATGTCGAAGCA
GAGGCAGAAGACAGGAATATTAGTATTTTAGGAGGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CHW1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References