Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | relBE/RHH(antitoxin) |
| Location | 5213..5772 | Replicon | plasmid unnamed1 |
| Accession | NZ_CP055076 | ||
| Organism | Shigella flexneri strain SWHIN_116 | ||
Toxin (Protein)
| Gene name | relE | Uniprot ID | V0SVC8 |
| Locus tag | HUZ90_RS22840 | Protein ID | WP_000421272.1 |
| Coordinates | 5213..5488 (-) | Length | 92 a.a. |
Antitoxin (Protein)
| Gene name | relB | Uniprot ID | V0SRR3 |
| Locus tag | HUZ90_RS22845 | Protein ID | WP_001387467.1 |
| Coordinates | 5488..5772 (-) | Length | 95 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HUZ90_RS22805 (HUZ90_22595) | 1263..2003 | - | 741 | WP_001066953.1 | tyrosine-type recombinase/integrase | - |
| HUZ90_RS22810 (HUZ90_22600) | 2124..2306 | - | 183 | WP_042344623.1 | hypothetical protein | - |
| HUZ90_RS22815 | 2373..2662 | - | 290 | Protein_3 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| HUZ90_RS22820 (HUZ90_22605) | 2649..2917 | - | 269 | Protein_4 | type II toxin-antitoxin system ParD family antitoxin | - |
| HUZ90_RS22825 (HUZ90_22610) | 3449..4618 | + | 1170 | WP_001072355.1 | hypothetical protein | - |
| HUZ90_RS22830 (HUZ90_22615) | 4651..4779 | + | 129 | Protein_6 | IS481 family transposase | - |
| HUZ90_RS22835 (HUZ90_22620) | 4814..5107 | - | 294 | WP_001105060.1 | hypothetical protein | - |
| HUZ90_RS22840 (HUZ90_22625) | 5213..5488 | - | 276 | WP_000421272.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| HUZ90_RS22845 (HUZ90_22630) | 5488..5772 | - | 285 | WP_001387467.1 | ribbon-helix-helix domain-containing protein | Antitoxin |
| HUZ90_RS22850 (HUZ90_22635) | 6377..7129 | + | 753 | WP_000562172.1 | IclR family transcriptional regulator | - |
| HUZ90_RS22855 (HUZ90_22640) | 7175..8140 | - | 966 | WP_001022265.1 | 2-keto-3-deoxygluconate transporter | - |
| HUZ90_RS22860 (HUZ90_22645) | 8173..8553 | - | 381 | WP_000710783.1 | Rid family detoxifying hydrolase | - |
| HUZ90_RS22865 (HUZ90_22650) | 8578..9468 | - | 891 | WP_001077068.1 | dihydrodipicolinate synthase family protein | - |
| HUZ90_RS22870 | 9701..9895 | - | 195 | WP_000796505.1 | hypothetical protein | - |
| HUZ90_RS22875 (HUZ90_22655) | 9869..10309 | + | 441 | Protein_15 | zinc-binding dehydrogenase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | tet(B) / catA1 / mph(A) / sul1 / qacE / aadA5 / blaTEM-1B / sul2 / aph(3'')-Ib / aph(6)-Id / aph(3')-Ia / aac(3)-IId / sitABCD | iutA / iucD / iucC / iucB / iucA | 1..125787 | 125787 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 92 a.a. Molecular weight: 10749.45 Da Isoelectric Point: 10.0871
>T161392 WP_000421272.1 NZ_CP055076:c5488-5213 [Shigella flexneri]
MELKWTSKALSDLSRLFDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYEINGQTIY
VLRLWHTRENR
MELKWTSKALSDLSRLFDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYEINGQTIY
VLRLWHTRENR
Download Length: 276 bp
>T161392 NZ_CP071150:4115586-4116119 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGTATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGTATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA
Antitoxin
Download Length: 95 a.a. Molecular weight: 10460.87 Da Isoelectric Point: 5.6558
>AT161392 WP_001387467.1 NZ_CP055076:c5772-5488 [Shigella flexneri]
MQMKNNAAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
SDSLSTDNPLPVPR
MQMKNNAAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
SDSLSTDNPLPVPR
Download Length: 285 bp
>AT161392 NZ_CP071150:4115309-4115575 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | V0SVC8 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0E0Y880 |