Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4200610..4201107 Replicon chromosome
Accession NZ_CP055075
Organism Shigella flexneri strain SWHIN_116

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag HUZ90_RS20610 Protein ID WP_001173343.1
Coordinates 4200610..4200783 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag HUZ90_RS20615 Protein ID WP_000398619.1
Coordinates 4200811..4201107 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ90_RS20600 (4197280) 4197280..4198737 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
HUZ90_RS20605 (4198974) 4198974..4200491 + 1518 WP_001295074.1 lysine--tRNA ligase -
HUZ90_RS20610 (4200610) 4200610..4200783 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
HUZ90_RS20615 (4200811) 4200811..4201107 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
HUZ90_RS20620 (4201334) 4201334..4201606 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
HUZ90_RS20625 (4201618) 4201618..4201848 - 231 WP_000371700.1 4Fe-4S mono-cluster protein YjdI -
HUZ90_RS20630 (4202029) 4202029..4203660 + 1632 WP_001216477.1 sensor histidine kinase -
HUZ90_RS20635 (4203657) 4203657..4204376 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T161391 WP_001173343.1 NZ_CP055075:c4200783-4200610 [Shigella flexneri]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T161391 NZ_CP071150:3998213-3998497 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGGTTGTGCAGAATCTGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCAGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT161391 WP_000398619.1 NZ_CP055075:c4201107-4200811 [Shigella flexneri]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT161391 NZ_CP071150:3997981-3998223 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References