Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3452666..3453284 Replicon chromosome
Accession NZ_CP055075
Organism Shigella flexneri strain SWHIN_116

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag HUZ90_RS16895 Protein ID WP_001291435.1
Coordinates 3453066..3453284 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag HUZ90_RS16890 Protein ID WP_000344800.1
Coordinates 3452666..3453040 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ90_RS16880 (3447755) 3447755..3448948 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
HUZ90_RS16885 (3448971) 3448971..3452120 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
HUZ90_RS16890 (3452666) 3452666..3453040 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
HUZ90_RS16895 (3453066) 3453066..3453284 + 219 WP_001291435.1 HHA domain-containing protein Toxin
HUZ90_RS16900 (3453456) 3453456..3454007 + 552 WP_000102564.1 maltose O-acetyltransferase -
HUZ90_RS16905 (3454123) 3454123..3454593 + 471 WP_000136192.1 YlaC family protein -
HUZ90_RS16910 (3454786) 3454786..3455483 + 698 WP_239635684.1 IS1-like element IS1A family transposase -
HUZ90_RS16915 (3455534) 3455534..3457084 + 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
HUZ90_RS16920 (3457126) 3457126..3457458 - 333 WP_001741293.1 DUF1428 family protein -
HUZ90_RS16930 (3457858) 3457858..3458169 + 312 WP_000409911.1 MGMT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T161386 WP_001291435.1 NZ_CP055075:3453066-3453284 [Shigella flexneri]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T161386 NZ_CP071150:1714606-1714708 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT161386 WP_000344800.1 NZ_CP055075:3452666-3453040 [Shigella flexneri]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT161386 NZ_CP071150:c1714715-1714570 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References