Detailed information of TA system TA161385

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 3080600..3081305
• Replicon: chromosome
• Accession: NZ_CP055075
• Organism: Shigella flexneri strain SWHIN_116

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: HUZ90_RS15095
• Protein ID: WP_000539521.1
• Coordinates: 3080600..3080986 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: HUZ90_RS15100
• Protein ID: WP_001280945.1
• Coordinates: 3080976..3081305 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ90_RS15075 (3076604)	3076604..3077230	+	627	WP_001295292.1	glutathione S-transferase GstB	-
HUZ90_RS15080 (3077227)	3077227..3078342	-	1116	Protein_2960	aldose sugar dehydrogenase YliI	-
HUZ90_RS15085 (3078453)	3078453..3078836	-	384	WP_000497137.1	biofilm formation regulator BssR	-
HUZ90_RS15090 (3079049)	3079049..3080374	+	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
HUZ90_RS15095 (3080600)	3080600..3080986	+	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HUZ90_RS15100 (3080976)	3080976..3081305	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
HUZ90_RS15105 (3081375)	3081375..3082703	-	1329	WP_000086877.1	GGDEF domain-containing protein	-
HUZ90_RS15110 (3082711)	3082711..3085059	-	2349	WP_001338403.1	EAL domain-containing protein	-
HUZ90_RS15115 (3085237)	3085237..3086148	-	912	WP_001236042.1	glutathione ABC transporter permease GsiD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T161385 WP_000539521.1 NZ_CP055075:3080600-3080986 [Shigella flexneri]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T161385 NZ_CP071150:1622742-1623074 [Klebsiella pneumoniae]
ATGGACAGAGGGGAAATCTGGCTTGTTTCACTGGATCCGATTGCTGGCCACGAGCAAAGTGGAAAACGTCCGGTACTCAT
TGTATCGAAGGCATCGTTTAACAAGCTGACCCGACTACCGGTCGTGGTTCCCGTCACCAGCGGCGGAAACTTTGCACGTA
CAGCAGGTTTCACGGTTTCACTGGAAGAGGCGGGGACCAAAACAACAGGCGTTATTCGCTGCGACCAGCCCAGGACTATC
GATATGGCGGCCCGGAAAGGCAAGCGTCTGGAACGCATACCTGACGTCGTTGTCAACGAGGTGCTTGCCAGGCTGGATGC
GATGCTGAGTTAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT161385 WP_001280945.1 NZ_CP055075:3080976-3081305 [Shigella flexneri]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT161385 NZ_CP071150:1622484-1622741 [Klebsiella pneumoniae]
ATGTATACAACTCGCCTGAAAAAGGTCGGCGGATCCATCATGCTGGCGGTTCCTCCCGCCGTGCTGAAAACGCTGGAGCT
GTCGACGGACAGCGAAGTGGGTATGACCATTGATAATGGCTGCCTGATTATTGAACCCCAGAAACGGCCCCGTTATTCGC
TTGAGGAGCTGCTGGCGCAGTGCGATCCGCACGCCGAAATGAGCGAAGAGGATCGGGAATGGATTGATGCGCCTGCGGTG
GGCAAGGAGATTCTGTAA

Structures

Toxin

• AlphaFold DB: A0A829GCT6