Detailed information of TA system TA161363

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/ElaA-DUF1778
• Location: 23354..24157
• Replicon: plasmid unnamed1
• Accession: NZ_CP055071
• Organism: Shigella flexneri strain SWHIN_97

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: G3CAI8
• Locus tag: HUZ91_RS23385
• Protein ID: WP_000348883.1
• Coordinates: 23627..24157 (+)
• Length: 177 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: G3CAI7
• Locus tag: HUZ91_RS23380
• Protein ID: WP_001275013.1
• Coordinates: 23354..23623 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ91_RS23360 (18671)	18671..20824	-	2154	WP_239606307.1	fimbria/pilus outer membrane usher protein	-
HUZ91_RS23365 (20840)	20840..21532	-	693	WP_001223973.1	molecular chaperone	-
HUZ91_RS23370 (21585)	21585..22128	-	544	Protein_20	fimbrial protein	-
HUZ91_RS23380 (23354)	23354..23623	+	270	WP_001275013.1	DUF1778 domain-containing protein	Antitoxin
HUZ91_RS23385 (23627)	23627..24157	+	531	WP_000348883.1	GNAT family N-acetyltransferase	Toxin
HUZ91_RS23390 (24158)	24158..24375	+	218	Protein_24	transposase	-
HUZ91_RS23395 (24431)	24431..24747	-	317	Protein_25	type II toxin-antitoxin system VapC family toxin	-
HUZ91_RS23400 (24744)	24744..24974	-	231	WP_001261274.1	type II toxin-antitoxin system VapB family antitoxin	-
HUZ91_RS23405 (24931)	24931..25029	+	99	Protein_27	hypothetical protein	-
HUZ91_RS23410 (25447)	25447..27711	-	2265	WP_015060475.1	UvrD-helicase domain-containing protein	-
HUZ91_RS23415 (27713)	27713..28489	-	777	WP_039052454.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	oqxA / oqxB / qnrS1 / sul3	-	1..144275	144275

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (92-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 177 a.a.
• Molecular weight: 20321.20 Da
• Isoelectric Point: 6.4889

>T161363 WP_000348883.1 NZ_CP055071:23627-24157 [Shigella flexneri]
MDGLRIEIFSEEVEYQLSNFDCGEEYLNTFLTDHLQRQHSSKILRGYLLVTREDRPRVMGYYTLSGSCFEKILLPSKTQQ
KRVPYKNVPSVTLGRLAIDKSIHHQGYGETLVTHAMKVVYQASQAVGIHGMFVEALNDNAKKFYLRLGFIQLKEENCDSL
FYPTKSIEVLFEVNDE

Nucleotide

• Length: 531 bp

>T161363 NZ_CP071137:c2416128-2415748 [Ferrovum myxofaciens]
ATGAGCGTACTCATCGACACCTCGGTGTGGATAGACCACTTCCGCAACGGAAATGATGCGTTGATTGACTTGATCGGGCT
AGACCTCGCGCTGACTCACCCAATGGTCATACTCGAAATTGCGTGCGGGACCCCCCCAGCGCCACGCATCCAGACACTGG
ATAACCTTGGCCTTTTGCAACCTTGTAACCAGGCGAGCATGCGTGAGGTAATGGAATTCATCGAGCGGGAAAAACTGTAT
GGGCTTGGGTGTGGGTTGGTGGACATGACACTACTGGCATCCACACTCATTACACCAGGTGCTGAGTTGTGGACTCTTGA
CAAGCGCCTTGCGGACTTGGCTGGGCGCTTCGGCGTGGCCCACCGGCCGACGCTGCATTAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10229.63 Da
• Isoelectric Point: 6.4702

>AT161363 WP_001275013.1 NZ_CP055071:23354-23623 [Shigella flexneri]
MSALKKQRIDLRLNEDDKHMIEEAAAMTNQSISQFMVSTASARAAEVIDQHRRLLLNEESWNLVMDAITNPPAPNDRLKR
ATNRLRELE

Nucleotide

• Length: 270 bp

>AT161363 NZ_CP071137:c2416331-2416125 [Ferrovum myxofaciens]
ATGCGTACCACTGTCACTATCGACGATGCACTCTACGAAAAGGCCCTGGAAATGGCCGACCCAAATATGGACAAATCGGA
TATTTTCCGAGAAGCAATCAAGACATTCGTGCGCGTGCAGGCAGCCAAGCGTCTTGCAACTCTAGGCGGCATGATACCCA
AGATTCAGGATGTACCGCGTCGCCGTGAGGAGCCAGTCGTGCAATGA

Structures

Toxin

• AlphaFold DB: G3CAI8

Antitoxin

• AlphaFold DB: G3CAI7