Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 4158301..4158896 Replicon chromosome
Accession NZ_CP055070
Organism Shigella flexneri strain SWHIN_97

Toxin (Protein)


Gene name chpB Uniprot ID U9Y4M4
Locus tag HUZ91_RS20630 Protein ID WP_000239579.1
Coordinates 4158301..4158651 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID U9Y2K1
Locus tag HUZ91_RS20635 Protein ID WP_001223208.1
Coordinates 4158645..4158896 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ91_RS20610 (4153747) 4153747..4154769 - 1023 WP_001295197.1 ABC transporter permease -
HUZ91_RS20615 (4154783) 4154783..4156285 - 1503 WP_000205784.1 sugar ABC transporter ATP-binding protein -
HUZ91_RS20620 (4156425) 4156425..4157381 - 957 WP_000265933.1 galactofuranose ABC transporter substrate-binding protein YtfQ -
HUZ91_RS20625 (4157691) 4157691..4158221 + 531 WP_000055072.1 inorganic diphosphatase -
HUZ91_RS20630 (4158301) 4158301..4158651 - 351 WP_000239579.1 endoribonuclease toxin ChpB Toxin
HUZ91_RS20635 (4158645) 4158645..4158896 - 252 WP_001223208.1 type II toxin-antitoxin system antitoxin ChpS Antitoxin
HUZ91_RS20640 (4159108) 4159108..4159449 - 342 WP_001219160.1 gamma-glutamylcyclotransferase -
HUZ91_RS20645 (4159452) 4159452..4163231 - 3780 WP_239605933.1 autotransporter assembly complex protein TamB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-114)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12511.45 Da        Isoelectric Point: 5.6219

>T161361 WP_000239579.1 NZ_CP055070:c4158651-4158301 [Shigella flexneri]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Download         Length: 351 bp

>T161361 NZ_CP071137:2403513-2403689 [Ferrovum myxofaciens]
ATGAACTCCAAGCAGATGAAGAAATGGCTAGAGCAACAGGGCGCGACTTTCCAGCCCGGTAAAGGCTCGCACCTGAAGGT
GTTTCTGAATGGCAAGCAGTCTTCACTCCCCATGCACGGTACCGCCGAACTTGGTAAAGGGCTGGAAGCCGCGATCAAAC
GACAGCTTGGTTTGTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9271.58 Da        Isoelectric Point: 4.4201

>AT161361 WP_001223208.1 NZ_CP055070:c4158896-4158645 [Shigella flexneri]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Download         Length: 252 bp

>AT161361 NZ_CP071137:2403703-2404122 [Ferrovum myxofaciens]
ATGATGTTCGACTACCCCGTGACCTTGACCCCCGATGACGGCACCATGCTGGTTACATTTGCCGATGTGCCCGAAGCCAT
CACCTTCGGTGCCGATGAGGATGAAGCCCTGCTGAACGCAATCGATGCGCTGGAAACGGGCTTGTCGTTTTATGTCGATG
CTCGCAAATCGCTACCCGTCGCCAGTCAGCCGGCGGCTGGACAAAAGACCGTGCGGCCATCGGCGCTGGAATGTGCCAAG
CTGGGCATTTACCAAGCGATGACCGATCAGGGAATCAAGAAGGCGGAACTCGCCCGCCGCTTGGGATGGCATATGCCGCA
AGTTGATCGCTTGTTCGATTTGCGTCATGCTTCAAAACTCGACCAGATCGAGGCTGCTGCCAACGTGCTCGGCAAACACG
TTTACGTGCAGATTGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y8A8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LQ26

References