Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4064227..4064803 Replicon chromosome
Accession NZ_CP055070
Organism Shigella flexneri strain SWHIN_97

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag HUZ91_RS20145 Protein ID WP_001297643.1
Coordinates 4064516..4064803 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A080I7H7
Locus tag HUZ91_RS20140 Protein ID WP_000063160.1
Coordinates 4064227..4064529 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ91_RS20125 (4060823) 4060823..4062973 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
HUZ91_RS20130 (4063023) 4063023..4063226 + 204 WP_000467859.1 YbdD/YjiX family protein -
HUZ91_RS20135 (4063237) 4063237..4064193 + 957 WP_001297640.1 GTPase -
HUZ91_RS20140 (4064227) 4064227..4064529 - 303 WP_000063160.1 BrnA antitoxin family protein Antitoxin
HUZ91_RS20145 (4064516) 4064516..4064803 - 288 WP_001297643.1 BrnT family toxin Toxin
HUZ91_RS24835 (4065002) 4065002..4065698 - 697 Protein_3954 IS1 family transposase -
HUZ91_RS20160 (4066013) 4066013..4067245 + 1233 WP_001137030.1 multidrug efflux MFS transporter MdtM -
HUZ91_RS20165 (4067286) 4067286..4068566 + 1281 WP_001037390.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4065002..4065211 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T161360 WP_001297643.1 NZ_CP055070:c4064803-4064516 [Shigella flexneri]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T161360 NZ_CP071137:c2285425-2285150 [Ferrovum myxofaciens]
ATGGAACTGAAGTGGACGAGCAAAGGGTTATCTGATTTGGCGCGGTTGTATGATTTCTTGGTGCCAGTGAACAAATCGGC
CGCTACGCGCGTGGTGCAGGCGCTCGCAGCGGCTCCAGCCAGTCTGCTGGTAAATCCACGTATCGGCGAAAAGCTGGAAG
AATTCGAGCCGCGCGAGGTACGCCGGATTCTCGTCGGAAAGTATGAGATGCGGTACGAAATTCGGGAATCCACGATCTAC
ATGCTGCGGCTGTGGCACACCCGCGAAGATCGGTAG

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11552.01 Da        Isoelectric Point: 9.4646

>AT161360 WP_000063160.1 NZ_CP055070:c4064529-4064227 [Shigella flexneri]
MSMVKHKRGTSSTLDVQHEAELKALTNKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT161360 NZ_CP071137:c2285694-2285425 [Ferrovum myxofaciens]
ATGGCACATACAGATACCAAAGTCCTCACTGCACACGTGCCGCTCCTGCTGGCCGAGAAAGTAGATCGGTTGGCCACTCG
TCTCGAACGCTCACGTGGCTGGATCGTCAAGCAAGCGTTAGCGGCCTGGATCGACATGGAAGAGGAACGCACCCGTTTGA
CCCACGAGGGGTTGGCCGATGTAGACGCCGGACGAGTCATTGATCACCAAGCCGTGGAAGCGTGGGCCAATAGCCTCAGT
ACTGCCCAACCGCTGCCGGCACCCCGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A080I7H7

References