Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3562102..3562720 Replicon chromosome
Accession NZ_CP055070
Organism Shigella flexneri strain SWHIN_97

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag HUZ91_RS17700 Protein ID WP_001291435.1
Coordinates 3562502..3562720 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag HUZ91_RS17695 Protein ID WP_000344800.1
Coordinates 3562102..3562476 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ91_RS17685 (3557191) 3557191..3558384 + 1194 WP_239605891.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
HUZ91_RS17690 (3558407) 3558407..3561556 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
HUZ91_RS17695 (3562102) 3562102..3562476 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
HUZ91_RS17700 (3562502) 3562502..3562720 + 219 WP_001291435.1 HHA domain-containing protein Toxin
HUZ91_RS17705 (3562892) 3562892..3563443 + 552 WP_000102564.1 maltose O-acetyltransferase -
HUZ91_RS17710 (3563559) 3563559..3564029 + 471 WP_000136192.1 YlaC family protein -
HUZ91_RS17715 (3564193) 3564193..3564585 + 393 Protein_3478 cyclic-guanylate-specific phosphodiesterase PdeB -
HUZ91_RS17725 (3565354) 3565354..3566520 + 1167 Protein_3480 cyclic-guanylate-specific phosphodiesterase PdeB -
HUZ91_RS17730 (3566562) 3566562..3566894 - 333 WP_001741293.1 DUF1428 family protein -
HUZ91_RS17740 (3567294) 3567294..3567605 + 312 WP_000409911.1 MGMT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T161357 WP_001291435.1 NZ_CP055070:3562502-3562720 [Shigella flexneri]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T161357 NZ_CP071137:c1162362-1162036 [Ferrovum myxofaciens]
ATGATGCGGGGTGATTTTGTAACCATTGTCATGCAAGGCGACTTTGGTAAACCAAGACCCGCCTTGGTAATTCAGGCCGA
TCAATTTGGCGAGCACGCAACTGTTACGGTGTTACCGATGACAAGCACGCTGGTTGCTGCTCCTTTGCTACGTACCCCTA
TTCAGCCAAGTGCCGAGAATGGCTTGAATAAGCCTTCGCAGGTGATGATTGACAAAGCCATGACCGTGAAGCGGGACAAG
TTGGGTCAGGCCTTTGGACGCATCGATGGGGTGGTGATGACGGAAGTTGAGCGTTGCCTGGCCGTGTTCTTGGGGATCGC
CAAGTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT161357 WP_000344800.1 NZ_CP055070:3562102-3562476 [Shigella flexneri]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT161357 NZ_CP071137:c1162580-1162359 [Ferrovum myxofaciens]
ATGGCAACACATGTGAATACACGAGTTCAGAGACACCGTGATACGTTGCGCATGGCTGGCCTGCGACCAATTCAAATTTG
GGTGCCGGATACACGACGGCCGGACTTCGCTGAGGAATGCCGCCGTCAGTCCCGCCTCGTCGCTCAGACGGACATGGTCG
ATACAGATCTGCAACACTTCATGGATGAAGCCTTGGCGGACATGGAAGGCTGGACGGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References