Detailed information of TA system TA161356

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 3120446..3121151
• Replicon: chromosome
• Accession: NZ_CP055070
• Organism: Shigella flexneri strain SWHIN_97

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: HUZ91_RS15390
• Protein ID: WP_000539521.1
• Coordinates: 3120446..3120832 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: HUZ91_RS15395
• Protein ID: WP_001280945.1
• Coordinates: 3120822..3121151 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ91_RS15370 (3116450)	3116450..3117076	+	627	WP_001295292.1	glutathione S-transferase GstB	-
HUZ91_RS15375 (3117073)	3117073..3118188	-	1116	Protein_3023	aldose sugar dehydrogenase YliI	-
HUZ91_RS15380 (3118299)	3118299..3118682	-	384	WP_000497137.1	biofilm formation regulator BssR	-
HUZ91_RS15385 (3118895)	3118895..3120220	+	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
HUZ91_RS15390 (3120446)	3120446..3120832	+	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HUZ91_RS15395 (3120822)	3120822..3121151	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
HUZ91_RS15400 (3121221)	3121221..3122549	-	1329	WP_000086877.1	GGDEF domain-containing protein	-
HUZ91_RS15405 (3122557)	3122557..3124905	-	2349	WP_001338403.1	EAL domain-containing protein	-
HUZ91_RS15410 (3125083)	3125083..3125994	-	912	WP_001236042.1	glutathione ABC transporter permease GsiD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T161356 WP_000539521.1 NZ_CP055070:3120446-3120832 [Shigella flexneri]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T161356 NZ_CP071131:908802-909623 [Caballeronia sp. M1242]
ATGTTCGATGCAAACGCGTTTCCATCGCGCTTCCTGAGCGGCATCGGCTATCGAGAAGCCGGCGGCGCGCACGCGGCGTC
GCTGCCAGTCGTGCTGCTGCACGGCATCGGCTCGGGCGCGGCGTCGTGGGTGCAGCAACTCGACGCGCTCGGCGCGACCC
GGCGCGTGCTCGCGTGGGACGCGCCGGGCTACGGCGAATCGGCGCCGGTCGCTTCGGCGTCACCGCAAGCCGCCGATTAC
GCCGATGCCTTGCGCGGCTGGCTCGACGCGCTCGATATCGAACGCTGCGTGCTGGTCGGCCATTCGCTCGGCGCGATTAT
CGCGGGCAGCTTCGCGGCGATGCATGAGAAGCGTGTCGCGGGCTTGCTGCTGCTGTCGCCGGCTGGCGGCTACGGCGCCG
CGAGCGAGGAGGAGCGCGCGCAAAAACGCGACAGCCGTCTCGCGATGCTCGCCGAACTCGGCCCGCAAGGACTCGCTGAT
CAACGCAGCGCGAACATGCTCTCGTCGAACGCGAGCGACGCGGCACGCGACTGGGTGCGCTGGAACATGGCGCGCATCGT
GCCGCAAGGCTATGCGCAGGCGACGCATCTGCTGGCGAATGCCGATCTCGCAAGCGATGTCGCGCGTTATCGCGGTCCGG
TGGCGGTGGCGGTCGGAACGGAGGATCGCATCACGACGCCGCAAGCGTGCGAGCGCATCGCGCAGGCGGCGGGCGTGCCG
CTGCGATTGGTCGAGGGCGCGGGCCACGCGGGTTACATCGAACAGCCCGATACGTATAACGCGCTCATCGGCTCATTCGC
GCAACAGGAGACGAAGGCATGA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT161356 WP_001280945.1 NZ_CP055070:3120822-3121151 [Shigella flexneri]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT161356 NZ_CP071131:909620-910453 [Caballeronia sp. M1242]
ATGACCCCGGCGGAAGAACAAGGCGACCTCACGTATCGCGTGCCCGGTCTCGAACGCGGCCTGCGCATCCTGAGCGAGTT
TTCGCCGCGCGAACCGGTGCTCGGCGCGCCGGAACTGTCGCGTCGTCTCGGCATTCCGCGCACGACGGTGTTCCGCCTGC
TGCAAACGCTGGAATCGCTCGGCTTTCTGGAGCGCGCCGACAGCGAGCGCAACTATCGGCTCGGCGTGGCGGTGCTGCGA
CTCGGCTTCGAATATCTGAGTTCGCTCGAACTGACGGATCACGGGCTGCCGCTTATCGAAGCGTTGCGCGATGACACCGG
CCTTTCGACGCACATCGTCATTCGCGATGGACGCGATGTAGTGTTCGTCGCCAAGGCGCAAAGCCACGCGAGCGGCTTCA
GCGCGGTGAAAGTCAACGTCGGCACGCGCCTGCCGGCGCACGCGACCACGCACGGCCACGTATTGATGGGCGATCTCAGC
CTCGACGACCTGCGCGAACTGTACGCCGGGCTCACCCTGGAGCGTTTCACGAGCCAGACGCCCGGCACAGTGGAAGAACT
GTTCGACCGCGTGAAGGTGGATGCCGAACGCGGCTACGCCACCAGCGAATCGTCGTTCGAACACGGCATTTCGGTCGTCA
GCGCGCCGGTGCGCGGCGACACCGGGCGCATTGCGGCGGTCGTCACCACGACGGTGCCACGCGCGCATATCGACACGACG
CTCATCGAAAGCGGACTCATCGACAAAGTGCGCGATACGGCTCATCAACTCTCGCTGCGGCTCAACTACCGGCCCGCCGC
GGAACATCGCTTTATGAAGGCATTGGGGCTTTGA

Structures

Toxin

• AlphaFold DB: A0A829GCT6