Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 857252..857906 Replicon chromosome
Accession NZ_CP055070
Organism Shigella flexneri strain SWHIN_97

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag HUZ91_RS04255 Protein ID WP_000244781.1
Coordinates 857499..857906 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag HUZ91_RS04250 Protein ID WP_000354046.1
Coordinates 857252..857518 (+) Length 89 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ91_RS04225 (852421) 852421..853164 + 744 WP_032298494.1 SDR family oxidoreductase -
HUZ91_RS04230 (853221) 853221..854654 - 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
HUZ91_RS04235 (854699) 854699..855010 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
HUZ91_RS04240 (855174) 855174..855833 + 660 WP_000250274.1 hemolysin III family protein -
HUZ91_RS04245 (856029) 856029..857009 - 981 WP_000886083.1 tRNA-modifying protein YgfZ -
HUZ91_RS04250 (857252) 857252..857518 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
HUZ91_RS04255 (857499) 857499..857906 + 408 WP_000244781.1 protein YgfX Toxin
HUZ91_RS04260 (857946) 857946..858467 - 522 WP_001055874.1 flavodoxin FldB -
HUZ91_RS04265 (858579) 858579..859475 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
HUZ91_RS04270 (859500) 859500..860210 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
HUZ91_RS04275 (860216) 860216..861949 + 1734 WP_000813198.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(3-131)

Antitoxin

(7-79)


Sequences


Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T161341 WP_000244781.1 NZ_CP055070:857499-857906 [Shigella flexneri]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T161341 NZ_CP071128:c2870118-2869723 [Mycobacterium tuberculosis]
ATGATCTTCGTCGACACGTCCTTCTGGGCTGCACTTGGCAACGCCGGCGACGCTCGGCACGGCACCGCGAAGAGGTTGTG
GGCCAGCAAACCGCCCGTGGTGATGACCTCCAACCATGTCCTGGGCGAGACCTGGACGCTGCTCAACCGGCGCTGCGGTC
ACCGCGCGGCGGTTGCCGCCGCTGCAATCCGCTTGAGCACCGTCGTTCGCGTCGAGCACGTAACAGCCGACCTGGAAGAG
CAGGCATGGGAATGGCTTGTACGCCACGACGAGCGAGAGTACTCGTTCGTCGATGCCACGAGCTTCGCGGTGATGCGAAA
GAAAGGCATCCAAAATGCGTATGCCTTCGATGGTGACTTCAGCGCGGCCGGATTTGTCGAGGTAAGACCCGAGTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT161341 WP_000354046.1 NZ_CP055070:857252-857518 [Shigella flexneri]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT161341 NZ_CP071128:c2870360-2870115 [Mycobacterium tuberculosis]
ATGCTAGTGGCGTATATTTGCCATGTGAAAAGGCTGCAGATCTACATCGACGAAGACGTTGACCGAGCGCTTGCTGTTGA
AGCGCGACGGCGGCGGACGTCGAAGGCGGCGCTGATCCGGGAGTACGTCGCTGAGCACCTCCGGCAGCCGGGCCCAGATC
CGGTCGATGCCTTCGTCGGATCGTTCGTGGGGGAAGCCGACTTGTCCGCGTCCGTCGACGACGTGGTTTACGGAAAGCAC
GAATGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB S1PAM6


Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References