Detailed information of TA system TA161337

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 36012..36655
• Replicon: plasmid unnamed2
• Accession: NZ_CP055068
• Organism: Klebsiella pneumoniae strain SWHE1

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: HUZ64_RS26950
• Protein ID: WP_239612300.1
• Coordinates: 36012..36428 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q84A07
• Locus tag: HUZ64_RS26955
• Protein ID: WP_001261276.1
• Coordinates: 36425..36655 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ64_RS26930 (HUZ64_26845)	32860..33906	+	1047	WP_239612298.1	hypothetical protein	-
HUZ64_RS26940 (HUZ64_26855)	35009..35695	+	687	Protein_32	IS5 family transposase	-
HUZ64_RS26945 (HUZ64_26860)	35741..35977	-	237	WP_064175067.1	hypothetical protein	-
HUZ64_RS26950 (HUZ64_26865)	36012..36428	-	417	WP_239612300.1	type II toxin-antitoxin system VapC family toxin	Toxin
HUZ64_RS26955 (HUZ64_26870)	36425..36655	-	231	WP_001261276.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
HUZ64_RS26960 (HUZ64_26875)	37237..37566	+	330	WP_069345704.1	hypothetical protein	-
HUZ64_RS26965 (HUZ64_26880)	37559..38245	+	687	WP_069345705.1	hypothetical protein	-
HUZ64_RS26970 (HUZ64_26885)	38242..39018	+	777	WP_069345706.1	site-specific integrase	-
HUZ64_RS26975 (HUZ64_26890)	39076..39333	-	258	WP_117326292.1	hypothetical protein	-
HUZ64_RS26980 (HUZ64_26895)	39457..39570	-	114	WP_168799920.1	hypothetical protein	-
HUZ64_RS26985 (HUZ64_26900)	40106..40972	+	867	WP_023302476.1	replication initiation protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	aadA2 / qacE / sul1	-	1..74918	74918

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15078.54 Da
• Isoelectric Point: 7.8644

>T161337 WP_239612300.1 NZ_CP055068:c36428-36012 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQVVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVN

Nucleotide

• Length: 417 bp

>T161337 NZ_CP071128:2808311-2808736 [Mycobacterium tuberculosis]
GTGGCGCTGCTCGACGTCAACGCATTGGTCGCGCTGGCGTGGGACTCACACATCCACCACGCCCGGATCCGCGAGTGGTT
TACCGCCAACGCCACGCTCGGCTGGGCGACTTGCCCGCTCACCGAAGCCGGCTTCGTGCGGGAGTCGACGAACCCAAAAG
TACTTCCCAGCGCGATCGGGATCGCAGACGCTCGACGGGTCCTCGTGGCACTACGCGCCGTGGGAGGCCACCGCTTCCTG
GCTGACGACGTATCGCTCGTCGATGACGATGTTCCGTTGATCGTCGGTTATCGCCAGGTGACCGACGCCCATCTGCTGAC
ACTCGCCCGCCGGCGCGGCGTCCGCCTGGTCACCTTCGACGCCGGTGTCTTCACCCTCGCCCAACAACGCCCCAAGACGC
CAGTGGAGCTGCTGACCATCCTCTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8721.74 Da
• Isoelectric Point: 4.3533

>AT161337 WP_001261276.1 NZ_CP055068:c36655-36425 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT161337 NZ_CP071128:2808084-2808305 [Mycobacterium tuberculosis]
GTGCGAACGACACTTGACCTTGACGACGATGTGATCGCCGCGGCACGTGAACTTGCCTCCAGCCAGCGCCGCTCGCTCGG
CTCGGTGATTTCCGAACTCGCACGCCGTGGTCTCATGCCCGGACGCGTCGAGGCTGACGACGGGCTGCCGGTGATCCGCG
TTCCAGCCGGGACCCCGCCGATCACACCGGAGATGGTCCGTCGCGCGCTCGATGAGGACTGA

Structures

Antitoxin

• AlphaFold DB: A0A387K023