161336

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5214723..5215348 Replicon chromosome
Accession NZ_CP055066
Organism Klebsiella pneumoniae strain SWHE1

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag HUZ64_RS25395 Protein ID WP_002882817.1
Coordinates 5214723..5215106 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag HUZ64_RS25400 Protein ID WP_004150355.1
Coordinates 5215106..5215348 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ64_RS25380 (5212089) 5212089..5212991 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
HUZ64_RS25385 (5212988) 5212988..5213623 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
HUZ64_RS25390 (5213620) 5213620..5214549 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
HUZ64_RS25395 (5214723) 5214723..5215106 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
HUZ64_RS25400 (5215106) 5215106..5215348 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
HUZ64_RS25405 (5215553) 5215553..5216470 + 918 WP_009484979.1 alpha/beta hydrolase -
HUZ64_RS25410 (5216484) 5216484..5217425 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
HUZ64_RS25415 (5217470) 5217470..5217907 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
HUZ64_RS25420 (5217904) 5217904..5218764 - 861 WP_004146232.1 virulence factor BrkB family protein -
HUZ64_RS25425 (5218758) 5218758..5219357 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T161336 WP_002882817.1 NZ_CP055066:c5215106-5214723 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T161336 NZ_CP071128:c2402507-2402190 [Mycobacterium tuberculosis]
ATGACGCGCAGGCTGCGCGTCCATAACGGGGTTGAAGACGACCTATTCGAGGCGTTTTCCTACTACGCGGACGCGGCGCC
AGATCAGATCGATCGGCTTTACAACTTGTTTGTCGATGCCGTGACGAAGCGGATTCCGCAGGCTCCGAACGCGTTTGCGC
CGTTATTCAAGCACTATCGACACATCTACCTCCGGCCCTTCAGGTACTACGTTGCCTATCGGACGACCGACGAGGCTATC
GACATACTGGCTGTTCGGCACGGAATGGAGAACCCGAACGCGGTCGAGGCTGAGATCTCTGGCCGCACCTTCGAGTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT161336 WP_004150355.1 NZ_CP055066:c5215348-5215106 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT161336 NZ_CP071128:c2402719-2402504 [Mycobacterium tuberculosis]
GTGGTGGTCAACCGGGCATTGCTGGCGAGCGTCGACGCACTGTCGCGTGATGAGCAGATTGAGCTCGTCGAGCACATCAA
CGGAAACCTAGCCGAGGGCATGCATATCAGCGAGGCCAACCAGGCGCTCATCGAAGCGCGGGCCAATGACACCGACGATG
CTCATTGGTCCACCATTGATGACTTCGACAAGCGGATCCGCGCCCGGCTCGGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References