Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3981279..3981898 Replicon chromosome
Accession NZ_CP055061
Organism Klebsiella pneumoniae strain SWHE3

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag HUZ66_RS19420 Protein ID WP_002892050.1
Coordinates 3981680..3981898 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag HUZ66_RS19415 Protein ID WP_002892066.1
Coordinates 3981279..3981653 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ66_RS19405 (HUZ66_19360) 3976431..3977624 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
HUZ66_RS19410 (HUZ66_19365) 3977647..3980793 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
HUZ66_RS19415 (HUZ66_19370) 3981279..3981653 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
HUZ66_RS19420 (HUZ66_19375) 3981680..3981898 + 219 WP_002892050.1 HHA domain-containing protein Toxin
HUZ66_RS19425 (HUZ66_19380) 3982061..3982627 + 567 WP_002892030.1 maltose O-acetyltransferase -
HUZ66_RS19430 (HUZ66_19385) 3982599..3982739 - 141 WP_004147370.1 hypothetical protein -
HUZ66_RS19435 (HUZ66_19390) 3982760..3983230 + 471 WP_002892026.1 YlaC family protein -
HUZ66_RS19440 (HUZ66_19395) 3983205..3984656 - 1452 WP_064145206.1 PLP-dependent aminotransferase family protein -
HUZ66_RS19445 (HUZ66_19400) 3984757..3985455 + 699 WP_004177238.1 GNAT family protein -
HUZ66_RS19450 (HUZ66_19405) 3985452..3985592 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
HUZ66_RS19455 (HUZ66_19410) 3985592..3985855 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T161311 WP_002892050.1 NZ_CP055061:3981680-3981898 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T161311 NZ_CP071128:c754992-754684 [Mycobacterium tuberculosis]
ATGCGGCGCGGTGAATTGTGGTTTGCCGCCACACCTGGTGGTGACAGACCAGTACTTGTCCTTACCAGAGATCCGGTGGC
AGACCGCATCGGCGCGGTCGTTGTGGTGGCCCTAACCCGCACCCGCCGAGGCCTGGTGTCGGAATTGGAGCTCACGGCCG
TCGAAAACCGTGTTCCGAGCGACTGCGTCGTCAACTTCGACAACATTCATACGTTGCCACGCACCGCATTCCGACGCCGC
ATCACCCGGCTGTCCCCGGCCCGCCTGCACGAAGCCTGTCAAACACTCCGGGCGAGCACGGGGTGTTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT161311 WP_002892066.1 NZ_CP055061:3981279-3981653 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT161311 NZ_CP071128:c755224-754979 [Mycobacterium tuberculosis]
ATGCTCAGCTTCCGCGCCGACGACCACGACGTCGACCTGGCCGACGCGTGGGCGCGTCGCCTACACATCGGCCGCTCCGA
GCTGCTGCGCGATGCGCTGCGACGTCACTTGGCCGCGCTGGCCGCGGACCAGGATGTCCAGGCCTACACCGAGCGACCGT
TGACGGACGACGAGAACGCACTGGCTGAGATCGCCGACTGGGGGCCGGCGGAGGACTGGGCCGACTGGGCCGATGCGGCG
CGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References