Detailed information of TA system TA161296

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): stbDE/ParE-YafN
• Location: 89662..90184
• Replicon: plasmid unnamed1
• Accession: NZ_CP055058
• Organism: Klebsiella pneumoniae strain SWHE4

Toxin (Protein)

• Gene name: stbE
• Uniprot ID: -
• Locus tag: HUZ67_RS26630
• Protein ID: WP_239613364.1
• Coordinates: 89662..89946 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: stbD
• Uniprot ID: -
• Locus tag: HUZ67_RS26635
• Protein ID: WP_161519102.1
• Coordinates: 89936..90184 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ67_RS26595 (HUZ67_26525)	84903..85532	-	630	Protein_95	Tn3 family transposase	-
HUZ67_RS26600 (HUZ67_26530)	85585..86289	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
HUZ67_RS26605 (HUZ67_26535)	86348..87781	-	1434	Protein_97	Tn3-like element Tn5403 family transposase	-
HUZ67_RS26610 (HUZ67_26540)	87876..88481	+	606	WP_000509966.1	recombinase family protein	-
HUZ67_RS26615 (HUZ67_26545)	88514..88806	-	293	Protein_99	transposase	-
HUZ67_RS26620 (HUZ67_26550)	89025..89375	-	351	WP_239613363.1	DUF1304 domain-containing protein	-
HUZ67_RS26625 (HUZ67_26555)	89490..89645	+	156	WP_239613369.1	hypothetical protein	-
HUZ67_RS26630 (HUZ67_26560)	89662..89946	-	285	WP_239613364.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HUZ67_RS26635 (HUZ67_26565)	89936..90184	-	249	WP_161519102.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
HUZ67_RS26640 (HUZ67_26570)	90702..92144	-	1443	WP_072439066.1	IS1182 family transposase	-
HUZ67_RS26645 (HUZ67_26575)	92405..92578	-	174	WP_045514387.1	addiction module toxin, GnsA/GnsB family	-
HUZ67_RS26655	94296..94382	+	87	WP_239613370.1	YnfU family zinc-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..236764	236764
	-	inside	IScluster/Tn	-	-	84903..117033	32130

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-84)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 11136.80 Da
• Isoelectric Point: 10.5787

>T161296 WP_239613364.1 NZ_CP055058:c89946-89662 [Klebsiella pneumoniae]
MTYKLAFNESALKEWKKLGHTVQEQFKKKLRERMENPRVPASQLHGRKDQYKIKLRGAGYRLVYRVEDDIVTVTVIGVGK
RENDDIYNVTRNRN

Nucleotide

• Length: 285 bp

>T161296 NZ_CP071127:3826540-3826950 [Mycobacterium tuberculosis]
GTGATCTATATGGACACCTCGGCCCTGACTAAGCTGCTCATCTCCGAGCCCGAGACGACCGAACTGCGGACATGGCTGAC
CGCGCAAAGCGGCCAGGGCGAGGACGCGGCGACAAGCACCCTTGGCCGGGTCGAGTAGATGAGAGTCGTTGCCCGATACG
GACAACCAGGCCAAACTGAGCGTGCGCGTTACCTACTCGACGGGCTCGACATCCTCCCGCTCACCGAACCGGTGATCGGT
CTAGCTGAAACGATCGGACCGGCCACCCTACGTTCTCTCGACGCGATTCACCTCGCGGCCGCAGCCCAGATCAAGCGGGA
ACTGACAGCCTTCGTCACCTACGACCACCGATTGTTGAGCGGATGCCGTGAGGTCGGCTTCGTCACCGCCTCACCCGGCG
CAGTCCGGTGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8952.20 Da
• Isoelectric Point: 3.8209

>AT161296 WP_161519102.1 NZ_CP055058:c90184-89936 [Klebsiella pneumoniae]
MAFQVLTTTAASITELKRDPMGTFNAGEGAPVAILNRNEPAFYCVPPALYAQLMEILEDQELGRIADERIGEAVIEVNID
DL

Nucleotide

• Length: 249 bp

>AT161296 NZ_CP071127:3826265-3826543 [Mycobacterium tuberculosis]
GTGGAGGCAATCGGAATCCGAGAACTAAGACAGCACGCATCGCGATACCTCGCCCGGGTTGAAGCCGGCGAGGAACTTGG
CGTCACCAACAAAGGAAGACTTGTGGCCCGACTCATCCCGGTGCAGGCCGCGGAGCGTTCTCGCGAAGCCCTGATTGAAT
CAGGTGTCCTGATTCCGGCTCGTCGTCCACAAAACCTTCTCGACGTCACCGCCGAACCGGCGCGCGGCAGCAAGCGCACC
CTGTCCGATGTTCTCAACGAAATGCGCGACGAGCAGTGA