Detailed information of TA system TA161295

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 34307..35217
• Replicon: plasmid unnamed1
• Accession: NZ_CP055058
• Organism: Klebsiella pneumoniae strain SWHE4

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A5Q9LR54
• Locus tag: HUZ67_RS26355
• Protein ID: WP_004181896.1
• Coordinates: 34747..35217 (+)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A2J4RLY9
• Locus tag: HUZ67_RS26350
• Protein ID: WP_004181895.1
• Coordinates: 34307..34750 (+)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ67_RS26330 (HUZ67_26265)	31372..31587	-	216	Protein_42	IS200/IS605 family transposase	-
HUZ67_RS26335 (HUZ67_26270)	31589..32158	+	570	Protein_43	transposase	-
HUZ67_RS26340 (HUZ67_26275)	32513..32935	+	423	WP_004181893.1	translesion error-prone DNA polymerase V autoproteolytic subunit	-
HUZ67_RS26345 (HUZ67_26280)	32935..34206	+	1272	WP_004181894.1	translesion error-prone DNA polymerase V subunit UmuC	-
HUZ67_RS26350 (HUZ67_26285)	34307..34750	+	444	WP_004181895.1	DUF2384 domain-containing protein	Antitoxin
HUZ67_RS26355 (HUZ67_26290)	34747..35217	+	471	WP_004181896.1	RES family NAD+ phosphorylase	Toxin
HUZ67_RS26360 (HUZ67_26295)	35327..35587	-	261	WP_004026352.1	hypothetical protein	-
HUZ67_RS26365 (HUZ67_26300)	36310..36468	+	159	WP_004181898.1	type I toxin-antitoxin system Hok family toxin	-
HUZ67_RS26370 (HUZ67_26305)	37071..38045	+	975	WP_040222313.1	hypothetical protein	-
HUZ67_RS26375 (HUZ67_26310)	38395..39363	-	969	WP_087728122.1	IS5 family transposase	-
HUZ67_RS26380 (HUZ67_26315)	39365..39685	-	321	WP_239613361.1	phosphonate ABC transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..236764	236764
	-	flank	IS/Tn	-	-	31805..32158	353
	-	flank	IS/Tn	-	-	38395..39363	968

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (30-89)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17549.81 Da
• Isoelectric Point: 4.6155

>T161295 WP_004181896.1 NZ_CP055058:34747-35217 [Klebsiella pneumoniae]
VIFYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T161295 NZ_CP071127:c3799627-3799235 [Mycobacterium tuberculosis]
ATGGCGGCCATCTATCTCGACTCGTCCGCGATCGTCAAGCTCGCGGTTCGCGAGCCAGAGTCGGACGCGCTGCGCCGCTA
CCTTCGCACCCGCCACCCGCGAGTGTCGAGTGCCCTTGCGCGCGCGGAGGTCATGCGGGCCCTGCTTGACAAGGGTGAAT
CAGCGCGGAAAGCCGGCCGCCGGGCGCTGGCTCATCTCGATCTGCTGCGCGTCGACAAGCGCGTCCTCGATCTCGCTGGT
GGGCTGCTGCCATTCGAGCTGCGCACGCTCGATGCCATCCACTTGGCGACGGCGCAGCGGCTCGGCGTCGATCTGGGCCG
GTTGTGCACCTACGACGACCGGATGCGCGACGCCGCCAAGACGCTCGGCATGGCCGTGATCGCCCCGTCGTGA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16536.87 Da
• Isoelectric Point: 10.3013

>AT161295 WP_004181895.1 NZ_CP055058:34307-34750 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVVNRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT161295 NZ_CP071127:c3799902-3799627 [Mycobacterium tuberculosis]
ATGACCTCGGTGGGGGTGCGTGCGCTGCGGCAGCGGGCCAGCGAGCTGCTGCGCCGAGTCGAGGCCGGCGAGACGATCGA
GATCACCGACCGCGGCCGGCCCGTCGCCCTGCTCTCGCCGCTGCCGCAGGGCGGTCCATACGAGCAGCTGCTGGCAAGCG
GCGAGATCGAGCGTGCCACGCTCGACGTCGTTGATCTGCCCGAACCGCTCGACCTCGACGCGGGCGTCGAGCTGCCGTCG
GTGACGCTTGCGCGCCTGCGTGAGCACGAGCGTTGA

Structures

Toxin

• AlphaFold DB: A0A5Q9LR54

Antitoxin

• AlphaFold DB: A0A2J4RLY9