Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4021789..4022408 Replicon chromosome
Accession NZ_CP055057
Organism Klebsiella pneumoniae strain SWHE4

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag HUZ67_RS19935 Protein ID WP_002892050.1
Coordinates 4022190..4022408 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag HUZ67_RS19930 Protein ID WP_002892066.1
Coordinates 4021789..4022163 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ67_RS19920 (HUZ67_19865) 4016941..4018134 + 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
HUZ67_RS19925 (HUZ67_19870) 4018157..4021303 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
HUZ67_RS19930 (HUZ67_19875) 4021789..4022163 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
HUZ67_RS19935 (HUZ67_19880) 4022190..4022408 + 219 WP_002892050.1 HHA domain-containing protein Toxin
HUZ67_RS19940 (HUZ67_19885) 4022571..4023137 + 567 WP_002892030.1 maltose O-acetyltransferase -
HUZ67_RS19945 (HUZ67_19890) 4023109..4023249 - 141 WP_004147370.1 hypothetical protein -
HUZ67_RS19950 (HUZ67_19895) 4023270..4023740 + 471 WP_002892026.1 YlaC family protein -
HUZ67_RS19955 (HUZ67_19900) 4023715..4025166 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
HUZ67_RS19960 (HUZ67_19905) 4025267..4025965 + 699 WP_094310543.1 GNAT family protein -
HUZ67_RS19965 (HUZ67_19910) 4025962..4026102 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
HUZ67_RS19970 (HUZ67_19915) 4026102..4026365 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T161290 WP_002892050.1 NZ_CP055057:4022190-4022408 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T161290 NZ_CP071127:3177814-3178077 [Mycobacterium tuberculosis]
GTGCCTTACACCGTGCGGTTCACCACAACCGCGCGTCGAGACCTCCACAAGCTGCCACCGCGAATCCTCGCGGCAGTGGT
CGAATTCGCGTTCGGCGATCTGTCGCGCGAGCCCCTGCGGGTGGGCAAGCCCCTTCGGCGCGAGTTGGCCGGCACGTTCA
GCGCGCGTCGCGGAACGTACCGCCTGCTGTACCGGATTGACGACGAGCACACAACGGTAGTGATCCTGCGCGTCGATCAC
CGCGCGGACATCTACCGCCGATAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT161290 WP_002892066.1 NZ_CP055057:4021789-4022163 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT161290 NZ_CP071127:3177529-3177810 [Mycobacterium tuberculosis]
ATGCGGATACTGCCGATTTCGACGATCAAGGGCAAGCTCAATGAGTTCGTCGACGCGGTCTCGTCGACACAGGACCAGAT
CACCATCACCAAGAACGGTGCACCCGCAGCCGTTCTGGTCGGCGCCGACGAGTGGGAATCGTTGCAGGAGACGCTGTACT
GGCTGGCGCAACCCGGAATCAGGGAGTCGATCGCTGAAGCCGACGCCGACATTGCCTCCGGCCGCACCTACGGCGAAGAC
GAGATCCGCGCCGAATTCGGCGTCCCGCGACGCCCCCACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References