Detailed information of TA system TA161275

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 198453..199192
• Replicon: chromosome
• Accession: NZ_CP055057
• Organism: Klebsiella pneumoniae strain SWHE4

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: HUZ67_RS00975
• Protein ID: WP_040226741.1
• Coordinates: 198707..199192 (+)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: HUZ67_RS00970
• Protein ID: WP_003026799.1
• Coordinates: 198453..198719 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ67_RS00955 (HUZ67_00950)	193957..196026	+	2070	WP_239613169.1	glycine--tRNA ligase subunit beta	-
HUZ67_RS00960 (HUZ67_00955)	196322..197691	+	1370	WP_087661561.1	IS3 family transposase	-
HUZ67_RS00965 (HUZ67_00960)	197892..198320	+	429	WP_004901287.1	GFA family protein	-
HUZ67_RS00970 (HUZ67_00965)	198453..198719	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
HUZ67_RS00975 (HUZ67_00970)	198707..199192	+	486	WP_040226741.1	GNAT family N-acetyltransferase	Toxin
HUZ67_RS00980 (HUZ67_00975)	199536..199688	+	153	WP_002922102.1	type I toxin-antitoxin system toxin HokA	-
HUZ67_RS00985 (HUZ67_00980)	199990..201608	+	1619	Protein_195	ATP-binding cassette domain-containing protein	-
HUZ67_RS00990 (HUZ67_00985)	201707..201919	-	213	WP_000014594.1	RNA chaperone/antiterminator CspA	-
HUZ67_RS00995 (HUZ67_00990)	202171..202461	-	291	WP_032412560.1	HTH-type transcriptional regulator	-
HUZ67_RS01000 (HUZ67_00995)	202707..204062	-	1356	WP_004901270.1	aromatic acid/H+ symport family MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	197059..197691	632

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (35-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17624.44 Da
• Isoelectric Point: 10.3370

>T161275 WP_040226741.1 NZ_CP055057:198707-199192 [Klebsiella pneumoniae]
VGRITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKKGTKQVAGFYSLATGSVNHTEATGSLRRNM
LDPIPVIILARLAVDVSLRGKGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKNFYIHHGFKPSQTQPRTLFLRLP
Q

Nucleotide

• Length: 486 bp

>T161275 NZ_CP071127:2321053-2321463 [Mycobacterium tuberculosis]
TTGGCTGAGCCACGGCGAGGAGACCTTTGGCTGGTCAGCCTCGGCGCCGCTCGCGCGGGTGAGCCCGGCAAGCATCGGCC
CGCGGTGGTCGTTTCCGTGGACGAGCTACTCACCGGAATCGACGACGAACTCGTTGTCGTCGTGCCGGTGTCAAGCTCGC
GCTCCCGCACCCCACTCCGGCCACCTGTCGCGCCCTCAGAAGGTGTAGCTGCCGATAGCGTCGCGGTGTGCCGCGGCGTC
CGCGCGGTCGCTCGTGCCCGACTCGTGGAGCGACTCGGCGCCCTCAAACCCGCCACGATGCGCGCAATCGAAAACGCCCT
GACCCTGATCCTCGGCCTCCCGACGGGACCTGAGCGCGGCGAGGCGGCGACCCATTCTCCCGTACGGTGGACGGGTGGCC
GGGACCCGTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT161275 WP_003026799.1 NZ_CP055057:198453-198719 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT161275 NZ_CP071127:2320827-2321060 [Mycobacterium tuberculosis]
ATGTCTACATCCACGACGATTAGGGTTTCAACCCAGACTCGGGATCGTCTGGCCGCCCAAGCCCGCGAACGGGGAATCTC
GATGTCGGCTCTGCTCACCGAACTGGCCGCCCAGGCCGAGCGCCAGGCAATCTTCCGCGCCGAACGCGAGGCCTCGCACG
CCGAGACGACCACCCAGGCAGTCCGCGACGAGGACCGCGAGTGGGAGGGCACGGTAGGCGACGGCCTTGGCTGA

Structures

Antitoxin

• AlphaFold DB: A0A1Q8YL66