Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 27815..28340 Replicon plasmid unnamed1
Accession NZ_CP055056
Organism Shigella dysenteriae strain SWHEFF_49

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag HUZ68_RS24315 Protein ID WP_001159871.1
Coordinates 27815..28120 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag HUZ68_RS24320 Protein ID WP_000813634.1
Coordinates 28122..28340 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ68_RS24285 (23231) 23231..24292 + 1062 WP_068858826.1 EAL domain-containing protein -
HUZ68_RS24290 (24700) 24700..24861 - 162 Protein_16 transposase -
HUZ68_RS24295 (24998) 24998..25291 - 294 Protein_17 integrase core domain-containing protein -
HUZ68_RS24300 (25597) 25597..26097 + 501 Protein_18 IS21-like element IS100 family transposase -
HUZ68_RS24305 (26094) 26094..26789 + 696 WP_074440817.1 AAA family ATPase -
HUZ68_RS24310 (27008) 27008..27814 - 807 WP_020219156.1 site-specific integrase -
HUZ68_RS24315 (27815) 27815..28120 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
HUZ68_RS24320 (28122) 28122..28340 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
HUZ68_RS24325 (29050) 29050..31671 + 2622 WP_020219157.1 histidinol-phosphatase -
HUZ68_RS24330 (32041) 32041..33036 + 996 WP_000246636.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..117256 117256


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T161269 WP_001159871.1 NZ_CP055056:c28120-27815 [Shigella dysenteriae]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T161269 NZ_CP071127:2201715-2202092 [Mycobacterium tuberculosis]
GTGCCGCCCCCTGATCCAGCCGCCATGGGTACCTGGAAGTTCTTCCGGGCATCTGTGGATGGCCGGCCGGTATTCAAGAA
GGAGTTCGACAAGCTTCCTGATCAGGCCCGGGCCGCGCTGATCGTGCTAATGCAGCGGTATCTCGTCGGCGACCTCGCCG
CAGGGAGCATCAAACCGATTCGTGGCGACATTCTGGAGTTGCGATGGCATGAGGCGAACAACCACTTCCGGGTACTGTTC
TTCCGCTGGGGCCAGCATCCCGTAGCGCTGACAGCGTTCTACAAGAACCAGCAGAAGACTCCCAAGACGAAGATCGAGAC
GGCCCTGGACCGGCAGAAAATCTGGAAAAGAGCCTTCGGCGACACCCCACCGATCTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT161269 WP_000813634.1 NZ_CP055056:c28340-28122 [Shigella dysenteriae]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT161269 NZ_CP071127:2202134-2202583 [Mycobacterium tuberculosis]
ATGAGCATTGACTTCCCTTTGGGTGACGACCTCGCCGGCTATATTGCCGAGGCGATTGCGGCTGATCCCAGCTTCAAAGG
CACTCTCGAAGACGCCGAGGAGGCACGCAGGCTGGTCGATGCGCTGATTGCGCTGCGCAAGCACTGCCAGCTGAGCCAGG
TTGAGGTTGCTAAGCGTATGGGGGTGCGCCAGCCCACCGTGAGCGGTTTCGAGAAGGAACCCAGCGACCCCAAACTGTCT
ACGCTGCAACGTTATGCCCGTGCATTGGACGCCCGGCTGCGGCTGGTGCTCGAAGTTCCCACGCTTCGCGAAGTGCCTAC
GTGGCATCGGCTCTCCTCTTATCGGGGCTCCGCACGGGACCAACAGGTCCGGGTGGGTGCAGACAAGGAAATCCTGATGC
AGACGAACTGGGCCCGCCACATTTCGGTTCGGCAGGTTGAGGTGGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References