Detailed information of TA system TA161254

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2291271..2291909
• Replicon: chromosome
• Accession: NZ_CP055055
• Organism: Shigella dysenteriae strain SWHEFF_49

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: HUZ68_RS10990
• Protein ID: WP_000813794.1
• Coordinates: 2291271..2291447 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: HUZ68_RS10995
• Protein ID: WP_077778300.1
• Coordinates: 2291493..2291909 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ68_RS10970 (2286890)	2286890..2288065	-	1176	WP_021556777.1	BenE family transporter YdcO	-
HUZ68_RS10975 (2288157)	2288157..2288693	+	537	WP_021577428.1	DNA-binding transcriptional regulator SutR	-
HUZ68_RS10980 (2288766)	2288766..2290727	+	1962	WP_033815235.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
HUZ68_RS10985 (2290819)	2290819..2291049	-	231	WP_000494244.1	YncJ family protein	-
HUZ68_RS10990 (2291271)	2291271..2291447	+	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
HUZ68_RS10995 (2291493)	2291493..2291909	+	417	WP_077778300.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
HUZ68_RS11000 (2291988)	2291988..2293394	+	1407	WP_000760655.1	PLP-dependent aminotransferase family protein	-
HUZ68_RS11005 (2293639)	2293639..2294784	+	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
HUZ68_RS11010 (2294802)	2294802..2295815	+	1014	WP_000220411.1	ABC transporter ATP-binding protein	-
HUZ68_RS11015 (2295816)	2295816..2296757	+	942	WP_001251315.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T161254 WP_000813794.1 NZ_CP055055:2291271-2291447 [Shigella dysenteriae]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T161254 NZ_CP071127:c755771-755334 [Mycobacterium tuberculosis]
ATGATAGTGCTCGACACCACCGTCCTCGTCTATGCCAAAGGCGCCGAGCATCCACTCCGCGATCCCTGCCGCGATCTCGT
CGCGGCAATCGCCGACGAACGCATCGCTGCGACCACCACCGCCGAGGTGATCCAGGAGTTCGTCCACGTACGCGCCCGCC
GGCGCGACCGCAGCGACGCCGCCGCGCTGGGCCGTGTGACTATGCCGAACTGCTCTCGCCGCTACTCACCATCAATCGAG
GCCACCTCGAAGCGCGGCCTGACGCTGTTCGAGACAACGCCAGGGCTTGAAGCCTGCGACGCGGTGCTCGCGGCGGTTGC
CGCAAGCGCCGGCGCGACCGCTCTTGTCTCGGCTGACCCGGCGTTCGCCGATCTGTCGGATGTCGTGCACGTCATTCCCG
ACGCGGCCGGGATGGTCAGCCTGCTCGGCGACCGCTGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15261.60 Da
• Isoelectric Point: 4.7386

>AT161254 WP_077778300.1 NZ_CP055055:2291493-2291909 [Shigella dysenteriae]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALQTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT161254 NZ_CP071127:c756022-755768 [Mycobacterium tuberculosis]
ATGAGCATGCGTCTGGCGCATCGGCTCCAGATCCTGCTCGACGACGAGTGCCACCGCCGCATCACCGCGGTGGCGCGCGA
GCGAGGCGTCCCGGTCGCAACCGTCGTGCGTGAGGCCATTGATCGAGGCCTCGTCAGCCCCGCCGGTCGCCGCAAGTCCG
CGGGCAGGCGCCTGCTCGACGCCGCCGACATGTCCGTTCCCGAGCCGCGGGAGCTGAAGCAGGAACTCGAGGCCCTCCGG
GCGCGCCGCGGATGA

Structures

Toxin

• PDB: 6HPB