Detailed information of TA system TA161253

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 1655142..1655847
• Replicon: chromosome
• Accession: NZ_CP055055
• Organism: Shigella dysenteriae strain SWHEFF_49

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A246P0D5
• Locus tag: HUZ68_RS07915
• Protein ID: WP_000539522.1
• Coordinates: 1655461..1655847 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: HUZ68_RS07910
• Protein ID: WP_001280945.1
• Coordinates: 1655142..1655471 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ68_RS07895 (1650299)	1650299..1651210	+	912	WP_001236044.1	glutathione ABC transporter permease GsiD	-
HUZ68_RS07900 (1651388)	1651388..1653736	+	2349	WP_000950335.1	EAL domain-containing protein	-
HUZ68_RS07905 (1653744)	1653744..1655072	+	1329	WP_033815368.1	GGDEF domain-containing protein	-
HUZ68_RS07910 (1655142)	1655142..1655471	-	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
HUZ68_RS07915 (1655461)	1655461..1655847	-	387	WP_000539522.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HUZ68_RS07920 (1656073)	1656073..1657398	-	1326	WP_033815369.1	30S ribosomal protein S12 methylthiotransferase RimO	-
HUZ68_RS07925 (1657611)	1657611..1657994	+	384	WP_000497137.1	biofilm formation regulator BssR	-
HUZ68_RS07930 (1658105)	1658105..1659220	+	1116	WP_021577174.1	aldose sugar dehydrogenase YliI	-
HUZ68_RS07935 (1659217)	1659217..1659843	-	627	WP_001295292.1	glutathione S-transferase GstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14292.51 Da
• Isoelectric Point: 10.2120

>T161253 WP_000539522.1 NZ_CP055055:c1655847-1655461 [Shigella dysenteriae]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIKDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T161253 NZ_CP071127:c755771-755334 [Mycobacterium tuberculosis]
ATGATAGTGCTCGACACCACCGTCCTCGTCTATGCCAAAGGCGCCGAGCATCCACTCCGCGATCCCTGCCGCGATCTCGT
CGCGGCAATCGCCGACGAACGCATCGCTGCGACCACCACCGCCGAGGTGATCCAGGAGTTCGTCCACGTACGCGCCCGCC
GGCGCGACCGCAGCGACGCCGCCGCGCTGGGCCGTGTGACTATGCCGAACTGCTCTCGCCGCTACTCACCATCAATCGAG
GCCACCTCGAAGCGCGGCCTGACGCTGTTCGAGACAACGCCAGGGCTTGAAGCCTGCGACGCGGTGCTCGCGGCGGTTGC
CGCAAGCGCCGGCGCGACCGCTCTTGTCTCGGCTGACCCGGCGTTCGCCGATCTGTCGGATGTCGTGCACGTCATTCCCG
ACGCGGCCGGGATGGTCAGCCTGCTCGGCGACCGCTGA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT161253 WP_001280945.1 NZ_CP055055:c1655471-1655142 [Shigella dysenteriae]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT161253 NZ_CP071127:c755224-754979 [Mycobacterium tuberculosis]
ATGCTCAGCTTCCGCGCCGACGACCACGACGTCGACCTGGCCGACGCGTGGGCGCGTCGCCTACACATCGGCCGCTCCGA
GCTGCTGCGCGATGCGCTGCGACGTCACTTGGCCGCGCTGGCCGCGGACCAGGATGTCCAGGCCTACACCGAGCGACCGT
TGACGGACGACGAGAACGCACTGGCTGAGATCGCCGACTGGGGGCCGGCGGAGGACTGGGCCGACTGGGCCGATGCGGCG
CGGTGA

Structures

Toxin

• AlphaFold DB: A0A246P0D5