Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 657707..658257 Replicon chromosome
Accession NZ_CP055055
Organism Shigella dysenteriae strain SWHEFF_49

Toxin (Protein)


Gene name ccdB Uniprot ID Q32K46
Locus tag HUZ68_RS03145 Protein ID WP_001160974.1
Coordinates 657943..658257 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag HUZ68_RS03140 Protein ID WP_000125566.1
Coordinates 657707..657940 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ68_RS03115 (652781) 652781..653068 + 288 WP_000203747.1 ferredoxin-like protein FixX -
HUZ68_RS03120 (653126) 653126..654457 + 1332 WP_001183199.1 MFS transporter -
HUZ68_RS03125 (654565) 654565..655095 + 531 WP_072686445.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
HUZ68_RS03130 (655088) 655088..656950 + 1863 WP_000377191.1 glutathione-regulated potassium-efflux system protein KefC -
HUZ68_RS03135 (657142) 657142..657621 + 480 WP_000624375.1 type 3 dihydrofolate reductase -
HUZ68_RS03140 (657707) 657707..657940 + 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
HUZ68_RS03145 (657943) 657943..658257 + 315 WP_001160974.1 CcdB family protein Toxin
HUZ68_RS03150 (658254) 658254..659102 - 849 WP_069913600.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
HUZ68_RS03155 (659109) 659109..659486 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
HUZ68_RS03160 (659489) 659489..660310 - 822 WP_001065381.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
HUZ68_RS03165 (660307) 660307..661296 - 990 WP_069913599.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
HUZ68_RS03170 (661296) 661296..662582 - 1287 WP_000800453.1 peptidylprolyl isomerase SurA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11737.84 Da        Isoelectric Point: 9.8517

>T161250 WP_001160974.1 NZ_CP055055:657943-658257 [Shigella dysenteriae]
MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFIRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T161250 NZ_CP071127:718281-718688 [Mycobacterium tuberculosis]
GTGAGCACGACGCCGGCCGCCGGAGTGCTCGACACGTCGGTGTTCATCGCGACCGAAAGCGGCCGGCAACTCGACGAGGC
GCTGATCCCCGACCGGGTCGCCACCACCGTCGTCACCCTCGCCGAACTGCGCGTCGGCGTGCTGGCCGCGGCGACGACCG
ACATCCGGGCTCAACGCCTGGCGACCCTGGAATCCGTTGCCGATATGGAAACGTTGCCCGTCGACGACGATGCCGCCCGA
ATGTGGGCCCGATTGCGGATCCATCTTGCCGAGTCCGGTCGCCGGGTGCGGATCAACGACCTGTGGATCGCGGCCGTCGC
GGCATCGCGAGCGCTGCCGGTCATCACCCAGGACGACGACTTCGCCGCCCTCGACGGTGCGGCCAGTGTGGAGATCATTC
GGGTCTGA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT161250 WP_000125566.1 NZ_CP055055:657707-657940 [Shigella dysenteriae]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT161250 NZ_CP071127:718024-718284 [Mycobacterium tuberculosis]
ATGTCTGAGGTGGCCTCGCGTGAGCTGCGTAACGATACGGCCGGCGTGCTGCGCCGCGTGCGGGCAGGGGAGGACGTCAC
CATCACCGTCAGCGGCCGTCCGGTCGCGGTGCTTACCCCGGTTCGTCCGCGGCGCCGGCGTTGGCTGAGCAAAACGGAGT
TCCTGTCGCGGTTGCGCGGCGCTCAAGCCGATCCCGGGCTCCGTAACGACCTCGCGGTCCTTGCCGGCGACACGACCGAG
GATCTCGGGCCGATCCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LRR3

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References