Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 320993..321490 Replicon chromosome
Accession NZ_CP055055
Organism Shigella dysenteriae strain SWHEFF_49

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag HUZ68_RS01635 Protein ID WP_001173343.1
Coordinates 321317..321490 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag HUZ68_RS01630 Protein ID WP_000398619.1
Coordinates 320993..321289 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ68_RS01610 (317724) 317724..318443 - 720 WP_000611288.1 two-component system response regulator DcuR -
HUZ68_RS01615 (318440) 318440..320071 - 1632 WP_001216477.1 sensor histidine kinase -
HUZ68_RS01620 (320252) 320252..320482 + 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
HUZ68_RS01625 (320494) 320494..320766 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
HUZ68_RS01630 (320993) 320993..321289 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
HUZ68_RS01635 (321317) 321317..321490 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
HUZ68_RS01640 (321609) 321609..323126 - 1518 WP_001295087.1 lysine--tRNA ligase -
HUZ68_RS01645 (323363) 323363..324820 - 1458 WP_023308599.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T161248 WP_001173343.1 NZ_CP055055:321317-321490 [Shigella dysenteriae]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T161248 NZ_CP071127:711005-711406 [Mycobacterium tuberculosis]
GTGACGGTGCTGCTCGACGCCAACGTGCTGATCGCATTGGTGGTCGCCGAGCATGTGCATCATGATGCCGCAGCGGACTG
GCTCATGGCGTCCGACACCGGATTTGCGACCTGCCCGATGACACAAGGAAGCCTGGTTCGATTCCTGGTGCGCTCGGGAC
AGTCCGCGGCGGCGGCTCGGGATGTCGTCAGTGCGGTCCAGTGCACGAGCCGCCACGAATTCTGGCCCGATGCACTCTCT
TTCGCCGGTGTCGAGGTCGCTGGTGTGGTTGGGCACCGGCAGGTGACCGATGCCTACCTTGCCCAGCTCGCGCGAAGCCA
CGACGGGCAGTTGGCGACGCTCGACAGCGGCTTAGCACACCTGCACGGCGACGTCGCGGTACTCATTCCAACGACCACCT
GA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT161248 WP_000398619.1 NZ_CP055055:320993-321289 [Shigella dysenteriae]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT161248 NZ_CP071127:710781-711008 [Mycobacterium tuberculosis]
GTGCGCACTACCATCGACCTGCCGCAAGACCTGCACAAGCAGGCACTGGCGATTGCCCGGGATACGCACCGCACGTTGAG
TGAAACGGTCGCCGACCTCATGCGACGAGGCCTGGCCGCCAACCGCCCTACCGCGTTGTCCTCAGACCCCAGAACGGGAT
TGCCTTTGGTGAGCGTCGGGACCGTCGTGACCTCCGAGGACGTGCGTTCATTAGAGGACGAGCAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References