Detailed information of TA system TA161215

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3976933..3977768
• Replicon: chromosome
• Accession: NZ_CP055048
• Organism: Escherichia coli strain SWHEFF_58

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A3L5HA82
• Locus tag: HUZ70_RS18980
• Protein ID: WP_061090001.1
• Coordinates: 3976933..3977310 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: HUZ70_RS18985
• Protein ID: WP_032256355.1
• Coordinates: 3977400..3977768 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ70_RS18950 (3973000)	3973000..3973983	-	984	WP_001298261.1	KpsF/GutQ family sugar-phosphate isomerase	-
HUZ70_RS18955 (3974794)	3974794..3974964	-	171	Protein_3697	IS110 family transposase	-
HUZ70_RS24690 (3975307)	3975307..3976148	-	842	Protein_3698	DUF4942 domain-containing protein	-
HUZ70_RS18970 (3976233)	3976233..3976430	-	198	WP_032256361.1	DUF957 domain-containing protein	-
HUZ70_RS18975 (3976448)	3976448..3976936	-	489	WP_061090000.1	DUF5983 family protein	-
HUZ70_RS18980 (3976933)	3976933..3977310	-	378	WP_061090001.1	TA system toxin CbtA family protein	Toxin
HUZ70_RS18985 (3977400)	3977400..3977768	-	369	WP_032256355.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HUZ70_RS18990 (3977818)	3977818..3978459	-	642	WP_061090002.1	hypothetical protein	-
HUZ70_RS18995 (3978478)	3978478..3978699	-	222	WP_000692303.1	DUF987 domain-containing protein	-
HUZ70_RS19000 (3978768)	3978768..3979244	-	477	WP_001186725.1	RadC family protein	-
HUZ70_RS19005 (3979260)	3979260..3979745	-	486	WP_061090003.1	antirestriction protein	-
HUZ70_RS19010 (3979836)	3979836..3980654	-	819	WP_016242967.1	DUF932 domain-containing protein	-
HUZ70_RS19015 (3980745)	3980745..3980978	-	234	WP_001117568.1	DUF905 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14047.00 Da
• Isoelectric Point: 7.8049

>T161215 WP_061090001.1 NZ_CP055048:c3977310-3976933 [Escherichia coli]
MNTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMARDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T161215 NZ_CP071100:c1523799-1523623 [Staphylococcus aureus]
TTGGATAGATGGTGGCTATCTGAGTATAAGGAGGTGGTGCCTATGTTGGCATTACTGAAATCTTTAGAAAGGAGACGCCT
AATGATTACAATTAGTACCATGTTGCAGTTTGGTTTATTCCTTATTGCATTGATAGGTCTAGTAATCAAGCTTATTGAAT
TAAGCAATAAAAAATAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13715.44 Da
• Isoelectric Point: 5.8808

>AT161215 WP_032256355.1 NZ_CP055048:c3977768-3977400 [Escherichia coli]
VSDSLHETNYPDDHNDRPWWGLPCTVTPCFGARLVQEGNQLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELSPRHQHTVTLYAKGLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT161215 NZ_CP071100:1523492-1523633 [Staphylococcus aureus]
ATATATAGAAAAAGGGCAACATGCGCAAACATGTTACCCTAGTGAGCCCGTTAAAAAGACGGTGGCTATTTTAGATTAAA
GATTAAATTAATAACCATTTAACCATCGAAACCAGCCAAAGTTAGCGATGGTTATTTTTTAT

Structures

Toxin

• AlphaFold DB: A0A3L5HA82