Detailed information of TA system TA161213

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 3301581..3302193
• Replicon: chromosome
• Accession: NZ_CP055048
• Organism: Escherichia coli strain SWHEFF_58

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: U9YXE2
• Locus tag: HUZ70_RS15695
• Protein ID: WP_000833473.1
• Coordinates: 3301581..3301766 (+)
• Length: 62 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: L4IWR9
• Locus tag: HUZ70_RS15700
• Protein ID: WP_000499744.1
• Coordinates: 3301783..3302193 (+)
• Length: 137 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ70_RS15685 (3298422)	3298422..3299573	+	1152	WP_137503999.1	L-threonine dehydrogenase	-
HUZ70_RS15690 (3299774)	3299774..3301312	+	1539	WP_001551836.1	aldehyde dehydrogenase AldB	-
HUZ70_RS15695 (3301581)	3301581..3301766	+	186	WP_000833473.1	type II toxin-antitoxin system HicA family toxin	Toxin
HUZ70_RS15700 (3301783)	3301783..3302193	+	411	WP_000499744.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
HUZ70_RS15705 (3302265)	3302265..3304229	-	1965	WP_001551835.1	glycoside hydrolase family 127 protein	-
HUZ70_RS15710 (3304240)	3304240..3305640	-	1401	WP_000204811.1	MFS transporter	-
HUZ70_RS15715 (3305866)	3305866..3306681	+	816	WP_000891823.1	AraC family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-129)

Sequences

Toxin

Protein

• Length: 62 a.a.
• Molecular weight: 6800.89 Da
• Isoelectric Point: 11.7053

>T161213 WP_000833473.1 NZ_CP055048:3301581-3301766 [Escherichia coli]
VKSADVIAILKQHGWEHIRTRGSHHQFRHPVHPGLVTVPHPKKDIKPGTLAQIGRQAGLTF

Nucleotide

• Length: 186 bp

>T161213 NZ_CP071099:2387140-2387394 [Fusobacterium animalis]
ATGTTATTGACTTGGACTGATTTTGCTTGGGAACAATATGAAGAATTACAAGAGAAAGATAAAAGACTTGTAAAAAAGAT
AAATACACTTATTAAGGATATCAAAAGAAATGATAATGAAGGAATAGGAAAACCAGAACCTTTACAACATGAACTAAGTG
GTTATTGGAGTAGAAGAATTGATGATAAAAATAGATTAGTTTATAAAATATCAGATACTCAAATAACAATAGTTGCTTGT
GCAAATCATTATTAA

Antitoxin

Protein

• Length: 137 a.a.
• Molecular weight: 15241.15 Da
• Isoelectric Point: 4.5486

>AT161213 WP_000499744.1 NZ_CP055048:3301783-3302193 [Escherichia coli]
MFYPAYIHSDPDGSASGFFPDVPGCYFAGDTLDNAFQDARSALVAHFETLCEMNEELPLPGSVETHLVQRAQDFIGGQWL
LVDINMNQFEGRAERINITMPKRLLNKIDTYVRNNPDYANRSAFLAEAARRVLPGV

Nucleotide

• Length: 411 bp

>AT161213 NZ_CP071099:2386883-2387140 [Fusobacterium animalis]
ATGATAGCAACAAATTATTCAGAGGTTAGAAATAATTTAAAAACTTATTGTGATAAGGCAACAAAGGACTATGAAACTAT
AATTATTACAAGAAAAAATAATGAAAATGTTGTTTTAATGAGTGAAGAAGAATATAACAATCTTATGGAAAACCTTTATA
TAAGATCAAATCTTAAATATTATCAAAGACTTGTAGAAAGTATAAAAGAAGCTGAAAAGGGTAATGTTAAAGAACATGAT
TTAATAGAGGTGGATTAA

Structures

Toxin

• AlphaFold DB: U9YXE2

Antitoxin

• AlphaFold DB: L4IWR9