Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 2642253..2642750 Replicon chromosome
Accession NZ_CP055048
Organism Escherichia coli strain SWHEFF_58

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag HUZ70_RS12735 Protein ID WP_001173343.1
Coordinates 2642253..2642426 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag HUZ70_RS12740 Protein ID WP_000398619.1
Coordinates 2642454..2642750 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUZ70_RS12725 (2638923) 2638923..2640380 + 1458 WP_001550473.1 dipeptide/tripeptide permease DtpC -
HUZ70_RS12730 (2640617) 2640617..2642134 + 1518 WP_001550472.1 lysine--tRNA ligase -
HUZ70_RS12735 (2642253) 2642253..2642426 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
HUZ70_RS12740 (2642454) 2642454..2642750 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
HUZ70_RS12745 (2642979) 2642979..2643251 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
HUZ70_RS12750 (2643263) 2643263..2643493 - 231 WP_001550470.1 4Fe-4S mono-cluster protein YjdI -
HUZ70_RS12755 (2643673) 2643673..2645304 + 1632 WP_001216477.1 sensor histidine kinase -
HUZ70_RS12760 (2645301) 2645301..2646020 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T161211 WP_001173343.1 NZ_CP055048:c2642426-2642253 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T161211 NZ_CP071097:999752-1000075 [Fusobacterium animalis]
GTGGTAGAAAGAGGAGATATTATCATAATAAATTTTGACCCTATAAAAAGACATGAACAAGTAGGGAAAAGACCAGTATT
AGTTATTAGTAATGAGAAATTCTATAAAATATTTAAACTAGCAGTAATTCTTCCAATAACAAATAATACAAAAGATTTTC
CATTTCATGTACTATTAGATAATAGAACAAATATAAAGGGTGCAATACTGTGTGAGTATTTAAAAACAGTTGATTTAGAA
GAAAGAAAATATAATAAAGTTGAAGAATTACCAGAAGATTTATTAGAAGAAGTCCTTGAAAAAGTGAGTTTAATTTTTCA
ATAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT161211 WP_000398619.1 NZ_CP055048:c2642750-2642454 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT161211 NZ_CP071097:999504-999758 [Fusobacterium animalis]
ATGGAAGTATTAAGAGTTCAAAAATGGGGAAATAGTCAAGGAATTAGATTACCTAAGAAAATTTTAAAAGACTTAGGAAT
AAATATAAATGATAAAATTGAAATAAGTTTAAATGGAGAAGAAATTATATTAAAAAAAATAAAAAAATATACTAATTTAG
ATGATTTGTTTAAAGACTATAAAGGAGATTATAGAAAAGATTTTGATGAATTTGAATTTTTTGGAGAAGCACAAGGTTGT
GAGTTTAAGTGGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References