Detailed information of TA system TA161206

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 1389637..1390342
• Replicon: chromosome
• Accession: NZ_CP055048
• Organism: Escherichia coli strain SWHEFF_58

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: HUZ70_RS06845
• Protein ID: WP_000539521.1
• Coordinates: 1389637..1390023 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: HUZ70_RS06850
• Protein ID: WP_001280945.1
• Coordinates: 1390013..1390342 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ70_RS06825 (1385641)	1385641..1386267	+	627	WP_001295292.1	glutathione S-transferase GstB	-
HUZ70_RS06830 (1386264)	1386264..1387379	-	1116	WP_000555051.1	aldose sugar dehydrogenase YliI	-
HUZ70_RS06835 (1387490)	1387490..1387873	-	384	WP_137503928.1	biofilm formation regulator BssR	-
HUZ70_RS06840 (1388086)	1388086..1389411	+	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
HUZ70_RS06845 (1389637)	1389637..1390023	+	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HUZ70_RS06850 (1390013)	1390013..1390342	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
HUZ70_RS06855 (1390412)	1390412..1391740	-	1329	WP_001550887.1	GGDEF domain-containing protein	-
HUZ70_RS06860 (1391748)	1391748..1394096	-	2349	WP_001550886.1	EAL domain-containing protein	-
HUZ70_RS06865 (1394274)	1394274..1395185	-	912	WP_001236042.1	glutathione ABC transporter permease GsiD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T161206 WP_000539521.1 NZ_CP055048:1389637-1390023 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T161206 NZ_CP071094:848820-849077 [Fusobacterium polymorphum]
ATGTTATTAACTTGGACCGATTTTGCATGGAAACAATATGAAGAGTTACAAGAAAAGGATAAAAGGCTTATAAAGAAGAT
AAATATACTTATTAAGGATATAAAAAGAAATGGAAATGAGGGAATAGGGAAACCAGAGCCTTTACAACATGAACTTAGTG
GTTATTGGAGCAGAAGAATTGATGATAAAAATAGATTAGTCTATAAAGTATCAGATAGTCAAATAATAATAGTTGCTTGT
GCAAATCATTATAAGTAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT161206 WP_001280945.1 NZ_CP055048:1390013-1390342 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT161206 NZ_CP071094:848563-848820 [Fusobacterium polymorphum]
ATGATAGCAACTAATTATTCTGAAGTTAGAAATAATTTAAAAGCTTATTGTGATAAGGCAACTAAGGACTATGAAATTAT
TATTATTACAAGAAAAAATAATGAAAATGTTGTTTTAATGAGTGAAGAAGAATATAATAATCTTATGGAAAACCTTTATA
TAAGATCAAATCTTAAATATTATCAAAAACTTGTAGAAAGTATAAAAGAAGTTGAAAAAGGTAATGTTAAAGAACATGAT
TTAATAGAGGTGGATTAA

Structures

Toxin

• AlphaFold DB: A0A829GCT6