Detailed information of TA system TA161204

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 751899..752537
• Replicon: chromosome
• Accession: NZ_CP055048
• Organism: Escherichia coli strain SWHEFF_58

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A7U9DHD1
• Locus tag: HUZ70_RS03725
• Protein ID: WP_000813795.1
• Coordinates: 752361..752537 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: HUZ70_RS03720
• Protein ID: WP_076797675.1
• Coordinates: 751899..752315 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ70_RS03700 (747051)	747051..747992	-	942	WP_001251315.1	ABC transporter permease	-
HUZ70_RS03705 (747993)	747993..749006	-	1014	WP_001551095.1	ABC transporter ATP-binding protein	-
HUZ70_RS03710 (749024)	749024..750169	-	1146	WP_001551094.1	ABC transporter substrate-binding protein	-
HUZ70_RS03715 (750414)	750414..751820	-	1407	WP_001551093.1	PLP-dependent aminotransferase family protein	-
HUZ70_RS03720 (751899)	751899..752315	-	417	WP_076797675.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
HUZ70_RS03725 (752361)	752361..752537	-	177	WP_000813795.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
HUZ70_RS03730 (752759)	752759..752989	+	231	WP_023910283.1	YncJ family protein	-
HUZ70_RS03735 (753081)	753081..755042	-	1962	WP_001551090.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
HUZ70_RS03740 (755115)	755115..755651	-	537	WP_001551089.1	DNA-binding transcriptional regulator SutR	-
HUZ70_RS03745 (755743)	755743..756915	+	1173	WP_001551088.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-65)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6747.80 Da
• Isoelectric Point: 11.5666

>T161204 WP_000813795.1 NZ_CP055048:c752537-752361 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPSEEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T161204 NZ_CP071094:c507151-506888 [Fusobacterium polymorphum]
ATGTATAAGGTATATTTTGACAAAAAAGTAGAAAAAGATTTTAAAAAATTAGATAAGAAAGTTTTAAAATTAATTTTAGA
TTGGATTGAAAATAATTTAGAAAATATAGAAGAACCAAGAAGTAAAGGAAAAGCATTGGTAGGAAATTTAAAGGATTATT
GGAGATATAGAATAGGTGATTATAGATTAATTACTAAAATTGATGATGGAAAACTATTAATTATTGCATTAGAACTTAAA
CATAGGAAAGAAGCCTATAAATAG

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15220.55 Da
• Isoelectric Point: 4.7386

>AT161204 WP_076797675.1 NZ_CP055048:c752315-751899 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPSNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT161204 NZ_CP071094:c507376-507152 [Fusobacterium polymorphum]
ATGTCAAACATTACATTAAGATTAACAGATGAAGAAAGAGAAATACTGAACAGTGTAGCTCATTTGTATGGTGATAAGTT
ATCAACAGCAATAAAAACAATATTATTTGAAAAAATAGAAGAAGATTATAATTTAAAAATAGTTAAAGATTTTGAAAAAA
GAGAAAAAGAAAATAAGGTGGAATTAGTTAGTTTATCTGATTTTAGAAAAAAGTTAGGTGTCTGA

Structures

Toxin

• AlphaFold DB: A0A7U9DHD1