Detailed information of TA system TA161203

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 652592..654180
• Replicon: chromosome
• Accession: NZ_CP055048
• Organism: Escherichia coli strain SWHEFF_58

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A829LC11
• Locus tag: HUZ70_RS03315
• Protein ID: WP_001551128.1
• Coordinates: 652858..654180 (+)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: HUZ70_RS03310
• Protein ID: WP_001296726.1
• Coordinates: 652592..652858 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUZ70_RS03310 (652592)	652592..652858	+	267	WP_001296726.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
HUZ70_RS03315 (652858)	652858..654180	+	1323	WP_001551128.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
HUZ70_RS03320 (654380)	654380..655732	-	1353	WP_001551127.1	SIR2 family protein	-
HUZ70_RS03325 (655947)	655947..656979	-	1033	Protein_654	ISNCY family transposase	-
HUZ70_RS03330 (657270)	657270..657530	+	261	Protein_655	aldo/keto reductase	-
HUZ70_RS03335 (657676)	657676..658371	+	696	WP_001552076.1	antibiotic biosynthesis monooxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	655947..656765	818

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-401)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49270.88 Da
• Isoelectric Point: 8.2472

>T161203 WP_001551128.1 NZ_CP055048:652858-654180 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLANRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTHPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGNVRALAVERFDRRW
NAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGAGIHISDLKLAMGVNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGIK

Nucleotide

• Length: 1323 bp

>T161203 NZ_CP071093:c696980-696723 [Fusobacterium vincentii]
ATGTTATTGACTTGGACTGATTTTGCATGGGAACAATATGAAGAATTACAGGAAAAAGATAAAAGACTTGTAAAAAAGAT
AAATATCCTTATTAAGGATATAAAAAGGAATGGTAATGAAGGAATAGGAAAACCAGAACCTTTACAACATGAGCTAAGTG
GCTATTGGAGTAGAAGAATTGATTATAAAAATAGATTAGTTTATAAGATATCAGATACTCAAATAATAATAGTTGCTTGT
GCAAATCATTATAAGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10047.39 Da
• Isoelectric Point: 4.9632

>AT161203 WP_001296726.1 NZ_CP055048:652592-652858 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDTKNASPES
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT161203 NZ_CP071093:c697237-696980 [Fusobacterium vincentii]
ATGATAGCAACTAATTATTCAGAGGTTAGAAATAATTTAAAAACTTATTGTGATAAGGCAACAAAGGACTATGAAACTAT
AATTATTACAAGAAAAAATAATGAAAATGTTGTCTTAATGAGTGAAGAAGAATATAACAATCTTATGGAAAACCTTTATA
TAAGATCAAATCTTAAATATTATCAAAAACTTGTAGAAAGTATAAAAGAAGTTGAAAAGGGTAATGTTAAAGAACATGAT
TTAGTAGAGGTGGATTAA

Structures

Toxin

• AlphaFold DB: A0A829LC11